HPLC测定芪肾口服液中黄芪甲苷和毛蕊异黄酮葡萄糖苷的含量
Determination of Astragaloside Ⅳ and Calycosin-7-O-β-D-glucopyranoside in Qishen Oral Liquid by HPLC
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摘要: 目的 建立高效液相法测定芪肾口服液中黄芪甲苷及毛蕊异黄酮葡萄糖苷的含量。方法 采用C18色谱柱，蒸发光散射检测器（ELSD）测定黄芪甲苷含量，ELSD条件：乙腈-水（32∶68）为流动相，漂移管温度102 ℃，载气流速2.7 L/min；采用紫外检测器（DAD）测定毛蕊异黄酮葡萄糖苷含量，DAD条件：乙腈-0.2%甲酸水为流动相梯度洗脱，检测波长260 nm。结果 黄芪甲苷、毛蕊异黄酮葡萄糖苷线性范围分别为1.1～16.5 μg，0.096～0.382 μg，平均加样回收率分别为96.73%（RSD=2.08%），100.70%（RSD=3.12%）。结论 该方法测定结果可靠，为含黄芪制剂质量标准建立提供参考。Abstract: OBJECTIVE To develop a HPLC method for the determination of astragaloside Ⅳ and calycosin-7-O-β-D-glucopyranoside in Qishen oral liquid. METHODS The astragaloside Ⅳ was determinated by ELSD with C18 column. Acetonitrile-water (ratio-32∶68) was set as the mobile phase， the temperature of drift tube was 102 ℃ and the gas flow rate was 2.7 L/min. DAD was used to detect calycosin-7-O-β-D-glucopyranoside， and the mobile phase was composed of acetonitrile and 0.2% formic acid aqueous for gradient elution with the detecting wave length of 260 nm. RESULTS The linear ranges of astragaloside Ⅳ and calycosin-7-O-β-D-glucopyranoside were 1.1～16.5 μg and 0.038～0.382 μg respectively. The average recoveries of two ingredients were 96.73% (RSD=2.08%) and 100.70% (RSD=3.12%). CONCLUSION The analytic results determined by HPLC were accurate， which can be used as reference for quality control in astragalus preparation.
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Key words:
- HPLC /
- astragaloside Ⅳ /
- calycosin-7-O-β-D-glucopyranoside
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[1] 简龙海，闻宏亮，夏晶，等.黄芪注射液定性定量方法的改进[J].中成药，2011，33(11):2018-2020. [2] Jian LH， Wen HL， Xia J， et al. Improvement of qualitative and quantitative methods for Astragalus membranaceus injection[J]. Chin Tradit Patent Med，2011，33(11):2018-2020. -
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