电针对ApoE-/-小鼠血浆hs-CRP、IL-6含量及主动脉窦斑块TNF-α表达的影响
Effect of Electroacupuncture on Content of hs-CRP and IL-6 in ApoE-/- Mouse Plasma and Expression of TNF-α in Arotic Sinus Plaques
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摘要: 目的 观察电针对ApoE-/-小鼠血浆超敏C反应蛋白（hs-CRP）、白介素-6（IL-6）含量及主动脉窦斑块肿瘤坏死因子-α(TNF-α)表达的影响。方法 将造模成功的ApoE-/-小鼠30只随机分为模型组和药物组及电针组，每组各10只。电针组予针刺单侧神门、内关、后三里及三阴交，并接通韩氏电针仪，药物组给予辛伐他汀40 mg/(kg·d)。采用ELISA法检测各组小鼠血浆hs-CRP及IL-6的含量，免疫组织化学法检测各组小鼠主动脉窦斑块中TNF-α的表达。结果 电针组血浆中hs-CRP、IL-6含量与模型组相比较，均有显著降低（P<0.01）；主动脉窦TNF-α免疫组化显示：模型组有大量TNF-α分布于纤维帽上，斑块中含有大范围坏死核和胆固醇结晶体，而电针组少量TNF-α表达于纤维帽上，未见明显坏死核和胆固醇结晶体。结论 电针治疗改善了ApoE-/-小鼠机体的炎症反应状态、达到保护血管内膜、抑制斑块发展的作用，说明抗炎可能是电针治疗AS的机制之一。Abstract: OBJECTIVE To observe the effect of electroacupuncture on the content of high sensitive C-reactive protein (hs-CRP) and interleukin-6 (IL-6) and the expression of tumor necrosis factor-a (TNF-α) in aortic sinus plaques in ApoE-/- mice plasma. METHODS 30 successfully-modeled ApoE-/- mice were randomly divided into three groups as model group， drug-control (DC) group and electro-acupuncture (EA) group， with 10 mice in each group. EA group was treated with acupuncture in Shenmen， Neiguan， Housanli and Sanyinjiao， connecting with the Hans electroacupuncture device. Meanwhile， the gavage administration of 40mg/kg.d simvastatin was adopted in DC group. The content of hs-CRP and IL-6 in mouse plasma was detected by ELISA and the expression of TNF-α was by immunohistochemistry (IHC). RESULTS Compared with model-group， the content of hs-CRP and IL-6 in EA group obviously decreased (P<0.01); IHC showed that there was plenty of TNF-α of model group distributed on fibrous cap and a large scale of necrotic core and cholesterol crystals was found in aortic sinus plaques; while in EA group， there was little TNF-α on fibrous cap without significant necrotic core and cholesterol crystals. CONCLUSION Electroacupuncture treatment can protect vessel endothelium and inhibit the development of plaques by improving the mice body's inflammatory response state， indicating that anti-inflammatory effect may be one of the mechanisms of AS treatment with electroacupuncture.
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Key words:
- electroacupuncture /
- atherosclerosis /
- ApoE-/- mice /
- hs-CRP /
- IL-6 /
- TNF-α
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