地耳草黄酮类成分UPLC-MS分析

陆黎; 韩乐; 邹立思; 刘训红

地耳草黄酮类成分UPLC-MS分析

1.
南京中医药大学附属南京市中医院，江苏南京 210001
2.
南京中医药大学药学院，江苏南京 210023

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出版历程
- 收稿日期: 2013-06-10
- 修回日期: 2013-07-04
- 刊出日期: 2013-09-10

Analytical Study on the Flavonoids Constituents in Herba Hyperici Japonici by UPLC-MS

1.
Nanjing Hospital of Traditonal Chinese Medicine, Nanjing, 210001, China
2.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的建立超高效液相色谱-质谱仪联用技术（UPLC-MS）对地耳草中黄酮类成分分析的方法。方法采用Alltima C₁₈色谱柱(5 μm，250 mm×4.6 mm，i.d.)，以乙腈-0.8%醋酸为流动相，梯度洗脱，流速为0.8 mL/min，检测波长为254 nm，柱温为30 ℃。在电喷雾负离子模式下监测采集数据。结果初步鉴定8种黄酮类化合物，并同时测定其中6种黄酮的含量。6种黄酮类化合物的浓度与峰面积的线性关系良好(r²>0.998 2)；加样回收率为97.6%~99.7%。结论该方法准确可靠，重现性好，可作为地耳草内在质量评价的依据。
- UPLC-MS /
- 地耳草 /
- 黄酮类
Abstract: OBJECTIVE To establish an Ultra Performance Liquid Chromatography (UPLC-MS) method to analyze the flavonoids constituents in Herba Hyperici Japonici.METHODS The analysis was performed on an Alltima C₁₈ chromatographic column with a gradient solvent system of acetonitrile-0.8% acetic acid at a flow rate of 0.8 mL/min. The detection wavelength was 254 nm and the column temperature was 30 ℃. The data was collected under the negative electrospray ion mode.RESULTS 8 flavonoids constituents being separated and identified， six of them were detected simultaneously. The result shows the detected flavonoids constituents have good linearity between concentration and peak area (r²>0.998 2)， and the average recoveries of the method were between 97.6%~99.7%.CONCLUSION Being simple， accurate and reproducible， UPLC-MS can be used for evaluating the quality of Herba Hyperici Japonici.
- UPLC-MS /
- Herba Hyperici Japonici /
- flavonoids constituents

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参考文献(5)

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