HPLC法同时测定半枝莲中黄酮和生物碱类成分
Simultaneous Determination of Flavones and Scutebarbatines in Scutellariae barbatae by HPLC
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摘要: 目的 建立同时测定半枝莲饮片中木犀草素、芹菜素、汉黄芩素、半枝莲生物碱A和半枝莲生物碱B的HPLC法。方法 采用高效液相色谱法,色谱柱为Phenomenon C18柱(250 mm×4.60 mm,5 μm),以乙腈-水溶液为流动相,梯度洗脱;流速为1.0 mL/min,柱温为30 ℃;木犀草素、芹菜素检测波长为355 nm,汉黄芩素、半枝莲生物碱A和生物碱B检测波长为264 nm。结果 木犀草素、芹菜素、汉黄芩素、半枝莲生物碱A和生物碱B分别在0.0374~2.40 μg、0.0312~2.00 μg、0.0312~2.00 μg、0.0062~0.40 μg和0.0062~0.40 μg内线性关系良好。精密度,重复性及加样回收均符合要求。结论 此方法快速简便,重现性好,可用于半枝莲饮片的质量控制。Abstract: OBJECTIVE To establish HPLC methods for simultaneous determination of luteolin, apigenin, wogonin, scutebarbatine A and scutebarbatine B.METHODS The analytical column was Phenomenex Luna C18(250 mm×4.60 mm, 5 μm). The mobile phase was composed of acetonitrile and water with gradient elution. The flow rate was 1.0 mL/min; The column temperature was 30 ℃. Luteolin and apigenin were detected at 355 nm; wogonin, scutebarbatine A and scutebarbatine B were detected at 264 nm.RESULTS The ranges of calibration curve of luteolin, apigenin, wogonin, scutebarbatine A and scutebarbatine B were 0.037 4~2.40 μg、0.031 2~2.00 μg、0.031 2~2.00 μg、0.006 2~0.40 μg and 0.006 2~0.40 μg, respectively. Also, the intra-batch and inter-batch variations and the average recoveries were all meet the requirement of Chinese Pharmacopoeia.CONCLUSION The method is rapid and simple with a good reproducibility and it can be used for the quality control of herba Scutellariae barbatae.
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Key words:
- Scutellariae barbatae /
- Flavones /
- Scutebarbatines /
- HPLC
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