痛泻要方对溃疡性结肠炎大鼠结肠黏膜ERK1、ERK2基因表达的影响

朱向东; 段永强; 王燕; 汪斌; 曹燕飞

痛泻要方对溃疡性结肠炎大鼠结肠黏膜ERK1、ERK2基因表达的影响

甘肃中医学院基础课部，甘肃兰州 730000

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出版历程
- 收稿日期: 2013-04-04
- 修回日期: 2013-05-14
- 刊出日期: 2013-07-10

Influence of Tongxie Yao Fang on ERK1 and ERK2 Gene Expressions of Colonic Mucosa in Rats with Ulcerative Colitis

摘要

摘要: 目的观察痛泻要方对溃疡性结肠炎（UC）模型大鼠结肠黏膜ERK1、ERK2基因表达的影响，探讨其作用机制。方法将实验动物分为6组，采用TNBS/乙醇灌肠法造模，肉眼观察结肠大体形态损伤并进行评分，以大鼠结肠黏膜ERK1、ERK2为观察指标，采用RT-PCR法检测ERK1、ERK2基因表达水平。结果肉眼观察模型组大鼠结肠组织黏膜层可见炎症和溃疡形成，与空白组比较，P<0.05，证实模型成功。模型组大鼠结肠组织ERK1、ERK2基因的相对表达量均高于空白组（P<0.05）；治疗后，痛泻要方高剂量组、中剂量组ERK1、ERK2基因的相对表达量均较模型组升高（P<0.05，P<0.01）。结论痛泻要方对TNBS/乙醇法UC大鼠模型结肠黏膜ERKl、ERK2基因的相对表达量有上调作用，提示痛泻要方治疗作用可能与ERK信号转导途径被激活有关。
- 痛泻要方 /
- 溃疡性结肠炎 /
- 基因表达
Abstract: OBJECTIVE To observe the influence of Tongxie Yao Fang on the ERK1 and ERK2 gene expressions of colonic mucosa in rats with ulcerative colitis （UC）， and to identify its underlying mechanism of action.METHODS Experimental animals were divided into six groups and prepared by means of TNBS/ ethanol enema to observe their morphological injury and damage with the naked eyes， and to detect ERK1 and ERK2 gene expressions by means of RT-PCR with ERK1 and ERK2 being the observation targets.RESULTS Inflammation and ulceration on the colonic mucous membrane of rats were observed with naked eyes， which suggested a successful modeling compared with the control group (P<0.05). The relative expression amount of ERK1 and ERK2 of colonic tissues of rats in the model group was higher than that of the blank group （P<0.05）; after treatment， compared with the model group， the relative expression amount of ERK1 and ERK1 in the prescription high- and moderate-dose groups were increased （P<0.05 and P<0.01）.CONCLUSION Tongxie Yao Fang can promote the relative expression amount of ERK1 and ERK2 of colonic tissues of rats with UC， which suggests the therapeutic effects of the prescription are probably related to the activation of ERK signal transduction pathway.
- Tongxie Yao Fang /
- ulcerative colitis /
- gene expression

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参考文献(12)

[1]	周红光，陈海彬，徐肇敏，等．溃克灵对溃疡性结肠炎大鼠模型结肠黏膜ICAM-1基因表达的影响[J]．南京中医药大学学报，2009， 25（3）：216-218．
[2]	Zhou HG， Chen HB， Xu ZM， et al. Effect of Kuikeling on ICAM-1 gene expression of colon mucosa of rats with ulcerative colitis[J]. J Nanjing Univ Tradit Chin Med， 2009， 25(3): 216-218.
[3]	陆玥琳，沈洪，张声生，等．中医序贯疗法对溃疡性结肠炎维持缓解的疗效观察[J]．南京中医药大学学报，2011， 27（2）：118-120．
[4]	Lu YL， Shen H， Zhang SS， et al. Effect of maintainable remission on ulcerative colitis with sequential therapy of traditional Chinese medicine[J]. J Nanjing Univ Tradit Chin Med， 2011， 27(2): 118-120.
[5]	Kidambi S， Yarmush J， Berdichevsky Y， et al. Propofol induces MAPK/ERK cascade dependant expression of cFos and Egr-1 in rat hippocampal slices[J]. BMC Res Notes， 2010， 3(2): 201.
[6]	Pereira A， Fink G， Sundram S. Clozapine-induced ERK1 and ERK2 signaling in prefrontal cortex is mediated by the EGF receptor[J]. J Mol Neurosci， 2009， 39(1-2): 185-198.
[7]	刘启．ERK和JNK信号转导通路与疾病关系的研究进展[J]．医学综述，2011， 17（11）：1637-1639．
[8]	LIU Q. Progress on the relationship between ERK， JNK signal transduction pathway and diseases[J]. Med Recapitulate， 2011， 17(11): 1637-1639.
[9]	张明林，许建明，梅俏，等．ERK-1和ERK-2蛋白表达与结肠癌组织中血管形成的关系[J]．安徽医学，2006， 27（ 03）：172-175．
[10]	Zhang ML， Xu JM， Mei Q， et al. Correlation of ERK-1 and ERK-2 protein expression on the angiogenesis of colonic carcinoma[J]. Anhui Med J， 2006， 27(03): 172-175.
[11]	宋宁，李燕舞，巫燕莉，等．溃结灵对UC大鼠结肠黏膜pERKl/2，pMEKl/2蛋白水平的影响[J]．中药新药与临床药理，2010， 21（2）：111-112．
[12]	Song N， Li YW， Wu YL， et al. Effect of Kuijieling Decoction on Protein Level of pMEK1/2 and pERK1/2 in Colonic Mucosa of Ulcerative Colitis Model Rats[J]. Tradit Chin Drug Res Clin Pharm， 2010， 21(2): 111-112.