四角蛤蜊酶解肽抑制ACE活性与抗氧化活性研究
Study on ACE-inhibitory and Antioxidant Activities of Mactra veneriformis Enzymatic Hydrolysis Peptides
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摘要: 目的 评价四角蛤蜊酶解肽体外抑制ACE活性与抗氧化活性。方法 以胃蛋白酶和胰蛋白酶模拟在体过程对四角蛤蜊进行酶解获得酶解肽。利用高效液相色谱法测定四角蛤蜊酶解肽的抑制ACE的活性,以Fenton法检测四角蛤蜊酶解肽对羟自由基的清除作用。结果 胰蛋白酶酶解6 h的四角蛤蜊酶解肽抑制ACE活性最佳,IC50=15.06 μg/mL。胰蛋白酶酶解2 h的羟自由基清除率最高,为93.78%。结论 四角蛤蜊酶解肽具有一定的抑制ACE活性与抗氧化活性。Abstract: OBJECTIVE To evaluate the ACE-inhibitory and antioxidant activities of Mactra veneriformis enzymatic hydrolysis peptides. METHODS Pepsin and trypsin were used to simulate enzymatic process in vivo,and then enzymatic hydrolysis peptides of Mactra veneriformis were obtained. The ACE inhibitory activity of Mactra veneriformis enzymatic hydrolysis peptides was measured by high performance liquid chromatography, and the radical scavenging effect was detected by Fenton system. RESULTS Mactra veneriformis enzymatic hydrolysis peptides was obtained by enzymatic hydrolysis of trypsin for 6h,which ACE-inhibitory activity of enzymatic hydrolysis peptides is the best, IC50=15.06 μg/mL. The best rate of hydroxyl radical scavenging of Mactra veneriformis enzymatic hydrolysis peptides is 93.78%,which was obtained by trypsin used for 2 h.CONCLUSION Mactra veneriformis enzymatic hydrolysis peptides have a definite ACE-inhibitory and antioxidant activity.
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Key words:
- Mactra veneriformis enzymatic hydrolysate /
- ACE-inhibitory /
- antioxidant
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