金欣口服液不同化学部位对呼吸道合胞病毒感染的影响
Experimental Study on Jinxin Oral Liquid Different Chemical Components Oppressing Respiratory Syncytial Virus
-
摘要: 目的 观察金欣口服液不同化学部位在体内的抗呼吸道合胞病毒（RSV）作用。方法 小鼠经滴鼻感染RSV建立肺炎模型，随机分为正常对照组、模型对照组、利巴韦林组、金欣口服液组、金欣口服液石油醚、乙酸乙酯、正丁醇提取液组及母液组，光镜下观察肺组织的炎症病变情况，计算小鼠肺指数和肺指数抑制率、脾指数、胸腺指数、死亡保护率。结果 金欣口服液石油醚、乙酸乙酯、正丁醇提取液对RSV感染后小鼠的肺指数增高均有显著的降低作用(P<0.01)，减轻感染小鼠肺组织炎症病变，同时能增加脾指数和胸腺指数(P<0.01)。金欣口服液正丁醇提取液能降低RSV感染小鼠的死亡率。结论 金欣口服液石油醚、乙酸乙酯、正丁醇提取液对RSV具有一定的抑制作用，并且能提高RSV感染小鼠的免疫功能。正丁醇提取液对RSV感染小鼠具有一定的死亡保护作用。Abstract: OBJECTIVE To observe the effect of Jinxin Oral Liquid (JOL) different chemical components on respiratory syncytial virus (RSV).METHODS The mice pneumonia models were set up through nasal drops with RSV， which were randomly divided into normal control group， model control group， ribavirin group， JOL group， petroleum ether extract group， ethyl acetate extract group， N-butanol extract group and mother liquor group. The condition of the inflammatory lesion in lung tissues was observed under light microscope. Pulmonary index and pulmonary index inhibition rate， spleen index， thymus index and death protection rate were calculated.RESULTS Petroleum ether extract， ethyl acetate extract and n-butanol extract of JOL remarkably decreased pulmonary index of mice infected with RSV (P<0.01) and reduced the inflammatory lesions in lung tissues; meanwhile， increased spleen index and thymus index (P<0.01). N-butanol extract of JOL reduced the death rate of mice infected with RSV. CONCLUSION Petroleum ether extract， ethyl acetate extract and n-butanol extract of JOL have a certain inhibitory effect on RSV， and can improve the immune function of mice infected with RSV. N-butanol extract of JOL can make certain death protection for mice with RSV.
-
Key words:
- Jinxin Oral Liquid /
- respiratory syncytial virus /
- pulmonary index /
- spleen index /
- thymus index
-
[1] 汪受传，朱先康，韩新民，等.小儿病毒性肺炎中医证治规律的初步研究[J].中国医药学报，2003，18（12）：694. [2] Wang SC， Zhu XK， Han XM， et al. Primary study on differentiation prescrition rules of children's viral pneumonia[J]. Chin J TCM & Pharm， 2003， 18(12): 694. [3] 白凌军，白凌鹰，汪受传，等.金欣口服液含药血清抗呼吸道合胞病毒作用研究[J].上海中医药大学学报，2009，23（6）：48-50. [4] Bai LJ， Bai LY， Wang SC， et al. Study on the effect of the serum contained Jinxin Oral Liquid anti-respiratory syncytial virus[J]. Acta Univ Tradit Med Sin Pharm Shanghai， 2009， 23(6): 48-50. [5] 李燕晖，李保刚. 中、重症呼吸道合胞病毒感染BALB/c小鼠模型的实验研究[J].昆明医学院学报，2009，30（4）：14-16. [6] Li YH， Li BG. The establishment of the moderate and severe BALB/c mouse model of RS virus infection[J]. J Kunming Med Univ， 2009，30(4): 14-16. [7] 李江全，任现志，汪受传. 清肺口服液对免疫功能影响的实验研究[J].中国实验方剂学杂志，2003，9（4）：47-48. [8] Li JQ， Ren XZ， Wang SC. Experimental study of Qingfei Oral Liquid on immune function[M]. Chin J Exper Tradit Med Form， 2003，9(4): 47-48. -
点击查看大图
计量
- 文章访问数: 712
- HTML全文浏览量: 5
- PDF下载量: 618
- 被引次数: 0
下载:
