影响ApoE<sup>-/-</sup>小鼠动脉病理变化因素的探讨

陶文剑; 詹冬梅; 艾炳蔚

影响ApoE^-/-小鼠动脉病理变化因素的探讨

南京中医药大学第二临床医学院，江苏南京 210023，2. 南京医科大学第一附属医院，江苏南京 210029，3. 南京中医药大学附属医院，江苏南京 210029

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出版历程
- 收稿日期: 2013-01-05
- 修回日期: 2013-02-17
- 刊出日期: 2013-03-10

Discussion on the Factors Affect Pathogenic Changes of ApoE^-/- Mice Artery

The Second Clinical School, Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的探讨不同脂肪和胆固醇含量饲料喂养对于早期ApoE^-/-小鼠动脉粥样硬化进展变化的影响。方法 8只6周龄ApoE^-/-小鼠随机分成2组，高脂高胆固醇饮食组（H组）4只，给予含21%脂肪、1.25%胆固醇的饲料喂养14周，中等脂肪饮食组（M组）4只，给予含10%脂肪的饲料喂养14周，观察比较不同脂肪和胆固醇含量饲料喂养后的血生化、Lee's指数、体脂比、肝体比和动脉病理切片的变化。结果 M组和H组的血清生化、Lee's指数、体脂比、肝体比无明显差异，常规HE染色切片、油红O冰冻切片显示20周的M组和H组主动脉都处于动脉粥样硬化脂质条纹期，斑块中表达大量的巨噬细胞源性泡沫细胞，存在大量坏死核和胆固醇结晶体，H组的主动脉窦上形成动脉瘤，M组腹主动脉形成动脉瘤，H组主动脉窦斑块中的胆固醇结晶体含量多于M组。结论 2组不同脂肪和胆固醇含量饲料对于早期ApoE^-/-小鼠动脉粥样硬化进程存在一定影响，但总体上变化不大。
- 动脉粥样硬化 /
- 动脉瘤 /
- 主动脉窦 /
- 饲料
Abstract: OBJECTIVE To explore the effect of different fat and cholesterol feedstuff on the ApoE^-/- mice atherosclerosis.METHODS Eight 6-week old mice were randomly put into two even groups. The High fat and cholesterol group (H group) were given 21% fat， 1.25% cholesterol feedstuff for 14 weeks， and the Middle fat and cholesterol group (M group) were given 10% fat for 14 weeks. Then observe the changes of blood biochemistry， Lee's index， and body fat percentage and Artery pathological section.RESULTS The results shows H group and M group have not much difference on the blood biochemistry， Lee's index， body fat percentage and Artery pathological section. Both the routine HE dyeing section， oil red O staining show the H group and M group were in atherosclerosis fatty streak period， and a lot of macrophage macrophage foam cells were expressed in the plague， a large amount of necrotic corn and cholesterol crystals were existed. H group had arterial aneurysm in the aortic sinus， and M group had arterial aneurysm in the aorta abdominalis， and the cholesterol crystals in the H group are much more than the M group.CONCLUSION Both the two groups of different fat and cholesterol feedstuff have effect on the ApoE^-/- mice atherosclerosis， but the influence is not that much.
- atherosclerosis /
- arterial aneurysm /
- aortic sinus /
- feedstuff

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参考文献(10)

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