当归饮子对复合型慢性荨麻疹致敏小鼠模型细胞因子的影响

郭静; 艾儒棣; 朱晓燕; 杜艾嫒; 钟振东

当归饮子对复合型慢性荨麻疹致敏小鼠模型细胞因子的影响

郭静^1,2,
艾儒棣^1,2,
朱晓燕^1,2,
杜艾嫒^1,2,
钟振东^3,

1.
成都中医药大学临床医学院，四川成都 610075
2.
成都中医药大学附属医院，四川成都 610075
3.
四川省人民医院四川省医学科学院实验动物研究所，四川成都 610072

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出版历程
- 收稿日期: 2012-10-12
- 修回日期: 2012-12-19
- 刊出日期: 2013-03-10

Danggui Yinzi's Effect on the Related Cytokines Abnormally Secreted by T cells of Chronic Urticaria belonging to Qi and Blood Deficiency Syndrome

GUOJing^1,2,
AIRu-di^1,2,
ZHUXiao-yan^1,2,
DUAi-yuan^1,2,
ZHONGZhen-dong^3
,

1.
Clinical Medical College, Chengdu University of Chinese Medicine, Chengdu, 610075, China
2.
The Affiliated Hospital, Chengdu University of Chinese Medicine, Chengdu, 610075, China
3.
Sichuan province people's hospital, Sichuan Academy of Medical Sciences Institute of Laboratory Animal, Chengdu, 610072, China

摘要

摘要: 目的研究当归饮子对气血两虚型慢性荨麻疹治疗机理。方法 KM小鼠，雌雄各半，采用利血平+乙酰苯肼，联合IV型变态反应模拟荨麻疹模型，选择模型制作成功的动物，随机分为模型组、氯雷他定组、当归饮子加减方高剂量组、中剂量组和低剂量组，每组8只动物，另取8只作为空白对照组。实验结束时，各组动物眼眶取血，分离血清，光镜检测外周血嗜酸性粒细胞（EOS）变化；采用双抗夹心ELISA法测试血清IgE含量；剖取血管壁组织，免疫组织化学检测血管壁组织中IL-4、IFN-γ表达量。结果与模型组比较，当归饮子各剂量组可显著降低动物外周血嗜酸性粒细胞（P<0.01）；降低血清IgE含量（P<0.01）；降低血管壁组织IL-4（P<0.01），升高IFN-γ（P<0.01）。结论当归饮子可通过干预T细胞异常分泌相关细胞因子抑制荨麻疹发病。
- 当归饮子 /
- 气血两虚型 /
- 荨麻疹 /
- 机理
Abstract: OBJECTIVE To study the therapeutic mechanism of Danggui Yinzi on chronic urticaria belonging to qi and blood deficiency syndrome.METHODS KM mice with equal number of male and female were given reserpine and acetophenone hydrazine to duplicate the model of qi and blood deficiency syndrome， and joint type IV allergic reaction imitated urticaria model. The successful models were selected and randomly divided into model group， loratadine group， high dose of modified Danggui Yinzi group， middle dose of modified Danggui yinZi group and low dose of modified Danggui Yinzi group， 8 animals for each， the rest 8 were taken only as control group. At the end of experiment， blood was taken from orbit of animals of each group， lens detected peripheral eosinophil (EOS) change after serum separation; serum IgE content was tested by ELISA; the expression quantity of hemal wall organization IL-4， IFN-γ was tested by immunohistochemical detection through cutting vascular wall organization.RESULTS Compared with the model group， Danggui Yinzi can significantly reduce the animal peripheral eosinophil in every dose group (P<0.01); It can reduce serum IgE content (P<0.01); It can reduce hemal wall organization IL-4(P<0.01)， It also can elevated IFN-V expression (P<0.01).CONCLUSION Danggui Yinzi can inhibit urticaria through intervening abnormal secretion of related cytokines in T cells.
- Danggui Yinzi /
- urticaria of qi and blood deficiency type /
- mechanism /

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参考文献(5)

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