留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

清肠化湿方对实验性大鼠结肠炎结肠黏膜上皮细胞紧密连接蛋白claudin-1的影响

翟金海 沈洪 倪菲菲 朱磊 刘智群

翟金海, 沈洪, 倪菲菲, 朱磊, 刘智群. 清肠化湿方对实验性大鼠结肠炎结肠黏膜上皮细胞紧密连接蛋白claudin-1的影响[J]. 南京中医药大学学报, 2013, 29(2): 151-154.
引用本文: 翟金海, 沈洪, 倪菲菲, 朱磊, 刘智群. 清肠化湿方对实验性大鼠结肠炎结肠黏膜上皮细胞紧密连接蛋白claudin-1的影响[J]. 南京中医药大学学报, 2013, 29(2): 151-154.
ZHAIJin-hai, SHENHong, NIFei-fei, ZHULei, LIUZhi-qun. Qingchang Huashi Fang's Influence on the Tight Junction Claudin-1 of the Colonic Mucosa Epithelial Cells Experimental Ulcerative Colitis Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2013, 29(2): 151-154.
Citation: ZHAIJin-hai, SHENHong, NIFei-fei, ZHULei, LIUZhi-qun. Qingchang Huashi Fang's Influence on the Tight Junction Claudin-1 of the Colonic Mucosa Epithelial Cells Experimental Ulcerative Colitis Rats[J]. Journal of Nanjing University of traditional Chinese Medicine, 2013, 29(2): 151-154.

清肠化湿方对实验性大鼠结肠炎结肠黏膜上皮细胞紧密连接蛋白claudin-1的影响

Qingchang Huashi Fang's Influence on the Tight Junction Claudin-1 of the Colonic Mucosa Epithelial Cells Experimental Ulcerative Colitis Rats

  • 摘要: 目的 观察清肠化湿方对实验性大鼠结肠炎结肠组织肿瘤坏死因子-α(TNF-α),紧密连接跨膜蛋白claudin-1表达水平的影响,以探讨其治疗溃疡性结肠炎(UC)的可能机制。方法 使用TNBS/无水乙醇造模成功后,分为空白组、模型组、清肠化湿方组12.8 g/(kg·d)、美沙拉嗪组(0.67 g/(kg·d)。一次性ig给药,连续10 d后,处死大鼠,留取结肠组织;ELISA法检测结肠组织TNF-a水平,免疫组化法观察结肠上皮claudin-1蛋白的表达情况,实时荧光定量PCR法检测claudin-1 mRNA相对表达水平。结果 清肠化湿方可以降低大鼠结肠炎模型结肠组织TNF-α水平[模型组,清肠化湿组分别为(99.40±32.37),(55.07±12.80)pg/mL],(P<0.01),提高claudin-1 mRNA相对表达水平[模型组,清肠化湿组分别为(2.18±0.78),(3.94±0.91)](P<0.01),减轻对claudin-1蛋白的损伤(P<0.05),清肠化湿方组与美沙拉嗪组疗效无明显差异。结论 清肠化湿方降低结肠组织TNF-α水平,保护紧密连接蛋白claudin-1,是其治疗UC的机制之一。

     

  • [1] Sappington PL, Han X, Yang R, et al. Ethyl pyruvate ameliorates intestinal epithelial barrier dysfunction in endotoxemic mice and immunostimulated caco-2 enterocytic monolayers[J]. J Pharmacol Exp Ther, 2003, 304(1): 464-476.
    [2] Bruewer M, Luegering A, Kucharzik T, et al. Proinflammatory cytokines disrupt epithelial barrier function by apoptosis-independent mechanisms[J]. J Immunol, 2003, 171(11): 6164-6172.
    [3] Ma TY, Iwamoto GK, Hoa NT, et al. TNF-alpha-induced increase in intestinal epithelial tight junction permeability requires NF-kappa B activation[J]. Am J Physiol Gastrointest Liver Physiol, 2004, 286(3): G367-G376.
    [4] Mankertz J, Waller JS, Hillenbrand B, et al. Gene expression of the tight junction protein occludin includes differential splicing and alternative promoter usage[J]. Biochem Biophys Res Commun, 2002, 298(5): 657-666.
  • 加载中
计量
  • 文章访问数:  693
  • HTML全文浏览量:  8
  • PDF下载量:  688
  • 被引次数: 0
出版历程
  • 收稿日期:  2012-10-22
  • 修回日期:  2012-12-26
  • 刊出日期:  2013-03-10

目录

    /

    返回文章
    返回