中药罗勒胶囊对大鼠移植性肝癌TACE后VEGF和Bcl-2表达的影响
Effects of Basil Capsules on Expressions of VEGF and Bcl-2 for Transplanted Liver Cancer in rats after TACE
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摘要: 目的 观察罗勒胶囊对大鼠移植性肝癌经肝动脉化疗栓塞术(TACE)后细胞中VEGF和Bcl-2表达的影响,探讨罗勒胶囊对肝肿瘤TACE后乏氧微环境的影响及意义。方法 建立大鼠移植性肝癌模型,10 d后将造模成功的大鼠随机分为5组。对TACE+生理盐水组和TACE+罗勒胶囊高、中、低剂量组4组大鼠进行TACE,并分别给予生理盐水10 mL/(kg·d)和罗勒胶囊300、150、75 mL/(kg·d) ig,对非TACE对照组大鼠只进行开腹操作,不进行TACE。给药10 d后取出移植性肝癌病理标本,免疫组化法检测肿瘤组织中VEGF和Bcl-2表达情况。结果 瘤质量:行TACE的4组大鼠与非TACE组比较均明显减轻(P<0.05,P<0.01),TACE+罗勒胶囊高剂量组较TACE+生理盐水组显著减轻(P<0.05);VEGF和Bcl-2阳性表达率:TACE+生理盐水组与非TACE组比均显著升高(P<0.01);TACE+罗勒胶囊高、中、低剂量组较TACE+生理盐水组显著降低(P<0.01);TACE+罗勒胶囊高剂量组较TACE+罗勒胶囊中、低剂量组显著降低(P<0.05,P<0.01)。结论 中药罗勒胶囊能改善肝肿瘤局部的乏氧微环境并促进细胞凋亡,使肿瘤组织中VEGF和Bcl-2表达降低,抑制大鼠移植性肝癌TACE术后肿瘤的侵袭和转移。
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关键词:
- 罗勒胶囊 /
- 大鼠移植性肝癌 /
- 经肝动脉化疗栓塞术 /
- 血管内皮生长因子 /
- 凋亡抑制基因Bcl-2
Abstract: OBJECTIVE To observe the effects of Basil capsules on expressions of VEGF and Bcl-2 for transplanted liver cancer in rats after transcatheter arterial chemoembolization (TACE) and to research the influence and significance of Basil capsules on hypoxia environment after TACE of rats hepatoma.METHODS Transplanted hepatoma models of rats were established. Ten days later, the successful models were divided into five groups randomly. The group with TACE and saline (A),group with TACE and high-dose Basil capsules (B), group with TACE and moderate-dose Basil capsules (C),group with TACE and low-dose Basil capsules (D) were injected with 10 mL/kg/d saline, 300 mg/kg/d/ Luole capsule, 150 mg/kg/d Basil capsule and 75 mg/kg/d Basil capsules respectively. The fifth group (E) as control group was operated just with laparotomy. After ten days of administration, the pathological specimen of transplanted liver cancer was taken out and the expressions of VEGF and Bcl-2 in tumor tissues were detected through immunohistochemistry.RESULTS As for the tumor weight, group A, B, C and D were obviously smaller than that of group E (P<0.01,P<0.05); group B was remarkably lighter than that of group A (P<0.05). As for the positive expression rate of VEGF and Bcl-2, both group A and E obviously increased (P<0.01);groups B, C and D visibly deceased compared with that of group A(P<0.01); groups B was remarkably lower than those of groups D and E (P<0.01,P<0.05). CONCLUSION Basil capsules can improve hypoxia environment in the local part of rat hepatoma, promote cell apoptosis, depress the expression of VEGF and Bcl-2 and inhibit the invasion and metastasis of tumor after TACE. -
[1] 王会杰,黄光明.肝癌TACE后残存病灶的影像学研究进展[J].中国实用医药,2011, 06(21):252-254. [2] Wang HJ, Huang GM.Progress in imaging of residual lesions in hepatocellular carcinoma after TACE[J]. Chin Prac Med, 2011, 06(21): 252-254. [3] 闵敏,高国兰.乏氧诱导因子相关研究进展[J].实用癌症杂志,2008, 23(4):424-426. [4] Min M, Gao GL.Progress in related study on HIF-α[J]. Prac J Can, 2008, 23(4): 424-426. [5] 孙艳,李琦.活血化瘀中药对癌转移影响的最新进展[J].实用医学杂志,2010, 26(9):1663-1665. [6] Sun Y, Li Q.Latest development of Chinese medicine for promoting blood circulation and removing blood stasis on carcinoma metastasis[J]. J Prac Med, 2010, 26(9): 1663-1665. [7] 郭文菁,曲迅,杨美香,等.罗勒多糖对荷NuTu-19卵巢癌大鼠模型的作用研究[J].中国病理生理学杂志,2006,22(8):1467-1470. [8] Guo WJ, Qu X, Yang MX, et al. Effect of basil polysaccharide on rat model bearing NuTu-19 cells[J]. Chin J Pathophysiol, 2006,22(8):1467-1470. [9] 朱峰,杨小伟,汤永辉,等.Walker-256大鼠移植性肝癌模型探讨[J].医学研究杂志,2010, 39(9):92-93. [10] Zhu F, Yang XW, Tang YH, et al.The establishment of transplantation model of liver cancer in Walker-256 tumor-bearing rats[J]. J Med Res, 2010, 39(9): 92-93. [11] 瞿雪梅,顾伟,胡佳娜,等.大鼠移植性肝癌模型血管介入的护理[J].实验动物科学与管理,2005, 22(2):56-57. [12] Qu XM, Gu W, Hu JN, et al. Nursing of the transarterial intervention experiment in transplannation hepatoma model of rats[J]. Lab Anim Sci & Man, 2005, 22(2): 56-57. [13] 乔连铭,冯敢生,侯继洲,等.RI与TACE联合栓塞对大鼠Walker-256肝移植模型肿瘤细胞增殖和凋亡的影响[J].中国实验诊断学,2007, 11(1):49-51. [14] Qiao LM, Feng GS, Hou JZ, et al.The effect of proliferaton and apoptosis in rat walker-256 hepatoma model after combination embolization with ribonuclease inhibitor plus TACE[J]. Chin J Lab Diag, 2007, 11(1): 49-51. [15] 俞武生,卢春丽,王在国,等.VEGF和MVD在术前接受过肝动脉栓塞化疗的肝癌组织中的表达及临床意义[J].中华普通外科学文献:电子版,2010, 04(4):323-327. [16] Yu WS, Lu CL, Wang ZG, et al. Expression and significance of VEGF and MVD in hepatocellular carcinoma patients received preop-erative transcatheter arterial embolization[J]. Chinese archives of general surgery: electronic version, 2010, 04(4): 323-327. [17] 吴行伟,曲恒燕,刘泽源.基于VEGF及其受体的抗肿瘤研究进展[J].中国药理通讯,2010, 27(4):42-42. [18] Wu HW, Qu HY, Liu ZY.Progress of anti-tumor study based on VEGF and its recepyor[J]. Chin Pharm, 2010, 27(4): 42-42. [19] Gang W, Zhao SP, Wu YZ, et al. Correlation of VEGF expression,angiogenesis and carcinomatous change in breast tumors[J]. The Chinese-German Journal of Clinical Oncology, 2010(7): 406-408. [20] Lichtenberger BM,Tan PK, Niederleithner H, et al. Autocrine VEGF signaling synergizes with EGFR in tumor cells to promote epithelial Cancer development[J]. Cell, 2010, 140(2): 268-279. [21] Thomadaki H, Scorilas A. BCL2 family of apoptosis-related genes: functions and clinical implications in Cancer[J]. Crit Rev Clin Lab Sci, 2006, 43(1): 1-67. [22] 陈健,高明太,蒋敏,等.血管内皮生长因子和bcl-2在肾母细胞瘤中的表达及意义[J].重庆医科大学学报,2010, 35(4):501-503. [23] Chen J,Gao MT, Jiang M, et al. Expression and significance of bcl-2 and vascular endothelial growth factor in nephroblastoma[J]. J Chongqing Med Univ, 2010, 35(4): 501-503. [24] O'donoghue GT, Pidgeon GP, Harmey JH, et al. Recombinant bactericidal permeability increasing protein (rBPI21) inhibits surgery-induced tumour growth in a murine model of metastatic disease[J]. Ir J Med Sci, 2008, 177(4): 359-365. [25] Chai L, Chen QE. Expression and significance of VEGF AND BCL-2 in oral squamous cell carcinoma[J]. Ch J histochemistry and cytochemistry, 2005, 14(3): 262-266. [26] Kroll J, Epting D, Kern K, et al. Inhibition of Rho-dependent kinases ROCK I/II activates VEGF-driven retinal neovascularization and sprouting angiogenesis[J]. Am J Physiol Heart Circ Physiol, 2009, 296(3): 893-899.
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