芡实提取物对SH-SY5Y神经细胞损伤的保护作用及体外抗氧化活性研究

沈蓓; 袁冬平; 伍城颖; 贺潇潇; 王新胜; 吴启南

芡实提取物对SH-SY5Y神经细胞损伤的保护作用及体外抗氧化活性研究

南京中医药大学药学院，江苏南京 210023

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出版历程
- 收稿日期: 2012-09-11
- 修回日期: 2012-12-11
- 刊出日期: 2013-01-10

The Study of Euryale Seed Extracts Protecting SH-SY5Y Neurocytes Damage and Their Invitro Antioxidant Activities

摘要

摘要: 目的观察芡实80%乙醇、乙酸乙酯、正丁醇提取物对过氧化氢（H₂O₂）诱导的SH-SY5Y神经细胞损伤的保护作用及其体外抗氧化活性。方法采用体外细胞培养的方法，建立SH-SY5Y神经细胞H₂O₂损伤模型。用Cell Counting Kit-8（CCK-8）试剂盒法检测以上不同浓度芡实提取物对H₂O₂诱导损伤的SH-SY5Y神经细胞活力的影响，计算抑制率。用体外抗氧化实验方法检测芡实3种提取物清除DPPH、ABTS自由基及还原铁的能力。结果芡实80%乙醇、乙酸乙酯、正丁醇提取物在含生药量10~30 mg/mL浓度范围内，均表现出明显的抑制H₂O₂对SH-SY5Y神经细胞氧化损伤的作用（P<0.05，P<0.01），且对DPPH、ABTS自由基及三价铁离子有很好的清除作用及还原能力，其中芡实正丁醇提取物的作用最强。结论芡实具有保护SH-SY5Y神经细胞免受H₂O₂氧化损伤的作用，有着很好的抗氧化活性，其中正丁醇提取物的抗氧化活性最强。
- 芡实提取物 /
- SH-SY5Y神经细胞 /
- H₂O₂诱导损伤 /
- 体外抗氧化
Abstract: OBJECTIVE To investigate the protective effects of euryale seed 80% ethanol， ethyl acetate and n-butyl alcohol extracts on the H₂O₂-induced damaged SH-SY5Y neurocytes and their invitro antioxidant activities.METHODS The H₂O₂-induced damaged SH-SY5Y neurocyte models were cultured outside of the body. Cell Counting Kit-8 (CCK-8) was used to determine the effects of the above euryale seed extracts with different concentrations on the activities of the H₂O₂-induced damaged SH-SY5Y neurocytes， and then their inhibition rates were calculated. The in-vitro antioxidant experimental method was applied to detecting the capabilities of the three extracts in removing DPPH， ABTS radicals and deoxidating three price iron ions.RESULTS Within the crude drug concentration range of 10~30mg/mL， euryale seed extracts in 80% ethanol， ethyl acetate and n-butyl alcohol showed obvious functions of inhibiting the oxidant damage action of H₂O₂ on the SH-SY5Y neurocytes (P<0.05， P<0.01). In addition， the above euryale seed extracts could markedly clear away DPPH， ABTS radicals and deoxidate three price iron ions， and among which， the n-butyl alcohol extract had the most powerful action.CONCLUSION Having an excellent antioxidant activity， euryale seed can protect the SH-SY5Y neurocytes from the damage of H₂O₂. Its active extracting solvents include ethanol， ethyl acetate and n-butyl alcohol. After a comprehensive comparison， it shows that the n-butyl alcohol extract has the most powerful function.
- euryale seed extracts /
- SH-SY5Y neurocyes /
- H₂O₂-induced damage /
- in-vitro antioxidant activity

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