板蓝根正丁醇部位对免疫细胞增殖及体外抗氧化活性的研究
Immune Cell Proliferation Function and in vitro Antioxidant Activity of n-butanol Extract of Radix Isatidis
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摘要: 目的 对板蓝根药材中提取的正丁醇部位各组分进行小鼠腹腔巨噬细胞和淋巴细胞体外增殖活性及体外抗氧化活性的研究。方法 从板蓝根中提取分离出12个正丁醇组分，采用HPLC法明确各组分在正丁醇部位色谱峰的归属。MTT法检测各组分对两种免疫细胞的增殖作用，同时采用清除DPPH、ABTS自由基和铁离子还原/抗氧化能力（FRAP）测定法，评价各组分的抗氧化活性。结果 2、6、7、11号组分对2种免疫细胞的增殖作用最强，最高增殖指数均大于3。2、6号组分的抗氧化活性最强，其清除DPPH自由基的IC50分别为0.366、0.729 mg/mL，清除ABTS自由基的IC50分别为0.164、0.338 mg/mL，对Fe3+还原性相当于1.441 mmol/g和0.812 mmol/g的FeSO4当量。结论 板蓝根正丁醇部位的提取物中存在促进免疫细胞增殖及抗氧化能力较强的活性成分，深入研究其结构，将有助于阐明板蓝根抗病毒药效物质基础。Abstract: OBJECTIVE To investigate proliferative activities for peritoneal macrophages and splenic lymphocytes in mice and anti-oxidative potency in vitro of n-butanol extract of Radix isatidis. METHODS 12 components were extracted and separated from Radix isatidis by n-butanol. Each component of total n-butanol extracts was detected and analyzed by HPLC. The proliferative activities of each component for peritoneal macrophages and splenic lymphocytes were detected by MTT assay. Anti-oxidative activities were evaluated by DPPH and ABTS radical scavenging and FRAP method. RESULTS No. 2， 6， 7 and 11 components showed the most potent proliferation on immune cells， with the highest proliferation index>3. No. 2 and 6 components displayed the strongest antioxidant activities， with IC50 values on DPPH of 0.391， 0.164 mg/mL， ABTS of 0.164， 0.338 mg/mL and Fe3+ reducibility values of 1.441， 0.812 mmol/g equivalent weight of FeSO4， respectively. CONCLUSION The n-butanol extract of Radix isatidis exhibit promoting proliferation for peritoneal macrophages and splenic lymphocytes and pontent antioxidant activities. Further investigation of the chemical structure will help to clarify the material basis for Radix isatidis antiviral effects.
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Key words:
- Radix isatidis /
- n-butanol /
- anti-oxidative activities /
- immunoenhancement
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