越鞠丸对帕金森病体外模型的神经保护作用研究

陈畅; 唐娟娟; 夏宝妹; 陈清; 王启盛; 陶伟伟; 赵杨; 陈刚

越鞠丸对帕金森病体外模型的神经保护作用研究

陈畅^1,2,
唐娟娟^1,,
夏宝妹¹,
陈清^1,2,
王启盛¹,
陶伟伟¹,
赵杨²,
陈刚^1,

1.
南京中医药大学基础医学院，江苏南京 210023
2.
南京中医药大学第三附属医院，江苏南京 210001

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出版历程
- 收稿日期: 2014-10-13
- 修回日期: 2015-01-28
- 刊出日期: 2015-03-10

The Neuroprotective Effects of Yueju Pills on Parkinson's Disease Model in vitro

1.
School of Basic Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210001, China

摘要

摘要: 目的研究越鞠丸醇提物（YJ-E）对1-甲基-4-苯基-1，2，3，6-四氢吡啶离子（MPP⁺）所致大鼠嗜铬细胞（PC12）损伤的保护作用，并探讨其作用机制。方法 PC12细胞给予1 mmol/L MPP⁺，制备帕金森病（PD）离体模型。应用四甲基偶氮唑盐（MTT）法检测细胞活力，Hoechst染色法观察细胞凋亡，观察YJ-E对PC12细胞的保护作用。应用Western blotting检测垂体腺苷酸环化酶激活肽（PACAP）的表达及细胞外调节蛋白激酶（ERK）、环磷腺苷效应元件结合蛋白（CREB）的磷酸化水平。结果 3个浓度1.0、2.0、4.0 mg/mL的YJ-E均能显著改善MPP⁺模型中PC12细胞的存活（P<0.01），而对正常PC12细胞的存活无显著影响（P>0.05）。同时YJ-E可显著上调MPP⁺模型中PC12细胞PACAP的表达（P<0.01），并且显著上调ERK、CREB的磷酸化（P<0.01）。结论 YJ-E对MPP⁺模型中的PC12细胞具有保护作用，其机制可能是通过上调PACAP的表达及其下游ERK、CREB的磷酸化。
- 越鞠丸醇提物 /
- 帕金森病 /
- 凋亡 /
- PACAP
Abstract: OBJECTIVE To investigate the neuroprotective effect and the mechanism of Yueju alcohol extract (YJ-E) on chromaffin cells (PC12) injury in rats induced by 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine ion (MPP⁺). METHODS PC12 cells were given 1 mmol MPP⁺ to establish Parkinson's disease (PD) model in vitro. The protective effects of YJ-E on PC12 cells were measured by MTT assay and Hoechst staining. The expression of pituitary adenylate cyclase activating polypeptide (PACAP) and the phosphorylation of extracellular regulated kinase (ERK) and cAMP response element binding protein (CREB) were determined by Western blotting. RESULTS YJ-E (1.0， 2.0， 4.0 mg/mL) improved the survival of MPP⁺-injured PC12 cells significantly (P<0.01)， and displayed no significant effect on normal PC12 cells (P>0.05). Meanwhile， YJ-E up-regulated the expression of PACAP and the phosphorylation of ERK and CREB significantly (P<0.01) of MPP⁺-injured PC12 cells. CONCLUSION YJ-E shows promising neuroprotective effects on PC12 cells in MPP⁺-induced Parkinson's disease model， and the mechanism may be associated with the up-regulation of the expression of PACAP and the phosphorylation of downstream ERK and CREB.
- Yueju alcohol extract /
- Parkinson's disease /
- apoptosis /
- PACAP

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参考文献(11)

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