四角蛤蜊粗多糖对四氧嘧啶诱导ICR小鼠糖尿病模型的降血糖作用
The Anti-hyperglycemic Activity of Crude Polysaccharides from Mactra Veneriformis in Alloxan Induced Diabetes in Mice
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摘要: 目的 研究四角蛤蜊粗多糖中具有降血糖作用的活性部位。方法 ICR小鼠，尾静脉注射四氧嘧啶(80 mg/kg）生理盐水溶液，96 h后筛选空腹血糖值≥11.1 mmol/L的糖尿病小鼠。四角蛤蜊粗多糖1按200、400、800、1 200 mg/kg剂量进行分组。分别测定第7、14天的空腹血糖值，糖化血清蛋白、总胆固醇及甘油三酯含量等指标。另取糖尿病小鼠，按照筛选出四角蛤蜊粗多糖1的最佳剂量（800 mg/kg），将四角蛤蜊粗多糖1按醇沉范围分段，相同生药量换算剂量，分为四角蛤蜊粗多糖2、3组。测定四角蛤蜊粗多糖1组（800 mg/kg）、四角蛤蜊粗多糖2组（180、360 mg/kg）和四角蛤蜊粗多糖3组（2 040 mg/kg）给药第7天空腹体质量和血糖水平。结果 四角蛤蜊粗多糖1具有降血糖作用，可降低小鼠糖化血清蛋白、总胆固醇及甘油三酯含量，其中四角蛤蜊粗多糖2（180、360 mg/kg）具有降血糖作用，四角蛤蜊粗多糖3无降血糖作用。结论 四角蛤蜊粗多糖1具有降血糖作用，最佳剂量为800 mg/kg，其降糖活性部位集中于四角蛤蜊粗多糖2。Abstract: OBJECTIVE To study the anti-hyperglycemic effective sites of crude polysaccharides from Mactra veneriformis (MVPS). METHODS Hyperglycemia models were established by intravenous injection of alloxan via tail vein of ICR mice under a dose of 80 mg/kg. After 96 h， mice with fasting blood glucose levels≥11.1 mmol/L were chosen and divided into groups. MVPS 1 treated at the doses of 200， 400， 800 mg/kg and 1 200 mg/kg once daily， respectively. At 7th and 14th days， respectively， the levels of blood glucose， GSP， TCH and TG were measured. Other diabetic mice were divided into MVPS 1 (800 mg/kg)， MVPS 2 (180， 360 mg/kg) and MVPS 3 (2 040 mg/kg) groups. After 7 d treatment， both body weights and fasting blood glucose levels were measured. RESULTS MVPS 1 of 800 mg/kg displayed the optimal anti-hyperglycemic effect on diabetic mice and reduced GSP， TCH and TG levels of diabetic mice; MVPS 2 showed significantly anti-hyperglycemic activities， compared to the model group. MVPS 3 exhibited no anti-hyperglycemic activities. CONCLUSION MVPS 1 displays the optimal hypoglycemic effect at the dose of 800 mg/kg， and its hypoglycemic active site focuses on MVPS 2.
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