电针对脑缺血再灌注模型大鼠海马IL-6受体mRNA表达的影响
Effect of Electroacupuncture on Interleukin-6 Receptor mRNA Expression in Hippocampus of Cerebral Ischemia-reperfusion Rats
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摘要: 目的 观察IL-6受体在脑缺血再灌注脑损伤中表达的变化,及电针对其表达的影响。方法 雄性SD大鼠随机分为假手术组、模型组、电针组,每组又各分为6 h和24 h观察组,各组每个时相各5只。采用改良Longa线栓法复制大脑中动脉局灶性脑缺血再灌注模型;电针组治疗取百会(DU20)及大椎(DU14)两穴,疏密波刺激30 min。应用qPCR法测定受损侧海马IL-6Rα mRNA及gp130 mRNA的表达。结果 IL-6Rα mRNA的表达随着时间的延长下降:在脑缺血再灌注6 h,模型组IL-6Rα mRNA表达最低,与假手术组相比有显著性差异(P<0.01),与电针组相比有显著性差异(P<0.05);在24 h时,模型组仍最低,与假手术组相比有显著性差异(P<0.01),与电针组相比无显著性差异,假手术组与电针组无显著性差异。IL-6信号转导子gp130 mRNA表达随着时间的延长上升:在脑缺血再灌注6 h,3组gp130 mRNA表达无显著性差异;在24 h时,假手术组表达无明显变化,模型组、电针组表达有所上升,电针组表达最高,与模型组相比有显著性差异(P<0.05),与假手术组相比有显著性差异(P<0.01),模型组与假手术组相比无显著性差异。结论 电针早期介入可以上调缺血再灌注脑组织的IL-6Rα mRNA、gp130 mRNA表达,这可能是电针发挥脑保护作用机制的一个途径。
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关键词:
- 脑缺血再灌注 /
- 电针 /
- IL-6Rα mRNA /
- gp130 mRNA
Abstract: OBJECTIVE To observe the changes of IL-6 receptor in hippocampus after cerebral ischemia-reperfusion and the effect on IL-6 receptor expression by electroacupuncture treatment. METHODS Sprague Dawley rats were randomly divided into sham-operation (sham), model and EA groups (five rats in each group). There are two observing time in each group: 6 hours and 24 hours after reperfusion. CI/R model was established by right middle cerebral artery occlusion for 2 h and reperfusion for 24 h. EA (3Hz, 1-3mA) was applied to "Baihui" (DU20), "Dazhui" (DU14) for 30 min. the expression of interleukin-6 receptor mRNA and gp130 mRNA in hippocampus by q-PCR method was observed. RESULTS ①The expression of interleukin-6Rα mRNA decreased as time changed: At the sixth hour, the expression of interleukin-6 Rα mRNA in the model group was the least compared with the sham group and EA group. There was significantly difference (P<0.01) compared with the sham group and (P<0.05) compared with the EA group; At the twenty-forth hour, the expression of interleukin-6 Rα mRNA of every group decreased, the expression of interleukin-6 Rα mRNA in the model group was still the least compared with the sham group (P<0.01), there was no significant difference between model group and EA group. ②The expression of gp130 mRNA increased as time changed; at the sixth hour, there was no difference in the three groups. At the twenty-forth hour, and the expression of gp130 mRNA in the sham group had no significant changes, the expression of gp130 mRNA of the model group and the EA group rose, the expression in the model group was less than that of the EA group significantly (P<0.05); while compared with the sham group, the expression of gp130 mRNA rose significantly (P<0.01) in EA group. CONCLUSION EA can up-regulate the expression of interleukin-6Ra mRNA and gp130 mRNA in rats' hippocampus of cerebral ischemia-reperfusion models. This may be one of the mechanisms of EA in protecting brain injury.-
Key words:
- cerebral ischemia-reperfusion /
- Electroacupuncture /
- IL-6 Rα mRNA /
- gp130 mRNA
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[1] Orion D, Schwammenthal Y, Reshef T, et al. Interleukin-6 and soluble intercellular adhesion molecule-1 in acute brain ischaemia[J]. Eur J Neurol, 2008, 15(4): 323-328. [2] Gravante G, Ong SL, Metcalfe MS, et al. Cytokine response to ischemia/reperfusion injury in an ex vivo perfused porcine liver model[J]. Transplant Proc, 2009, 41(4): 1107-1112. [3] 穆艳云,沈梅红,程洁,等.电针对脑缺血再灌注模型大鼠海马IL-6表达影响的观察[J].辽宁中医杂志,2013, 40(4):797-800. [4] Mu YY, Shen MH, Cheng J, et al.Observation of electroacupuncture on the IL-6 expression in cerebral infraction model[J]. Liaoning J Tradit Chin Med, 2013, 40(4): 797-800. [5] 沈梅红,李成,李忠仁.电针对脑缺血再灌注模型大鼠的GSH含量、GSH-Px及GR活性的影响[J].南京中医药大学学报,2011, 27(2):137-139. [6] Shen MH, Li C, Li ZR. Effects of electro-acupuncture on GSH,GSH-Px and GR of cerebral ischemia-reperfusion model rats[J]. J Nanjing Univ Tradit Chin Med, 2011, 27(2): 137-139. [7] Kuluz JW, Prado RJ, Dietrich WD, et al. The effect of nitric oxide synthase inhibition on infarct volume after reversible focal cerebral ischemia in conscious rats[J]. Stroke, 1993, 24(12): 2023-2029. [8] 李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:255-257. [9] Li ZR. Experimental acupuncture[M]. Beijing: China press of traditional Chinese medicine, 2003: 255-257. [10] Garcia JH, Wagner S, Liu KF, et al. Neurological deficit and extent of neuronal necrosis attributable to middle cerebral artery occlusion in rats Statistical validation[J]. Stroke, 1995, 26(4): 627-635. [11] 张洋,李元.白细胞介素6受体研究的新进展[J].医学研究通讯,2004,33(1) :37-39. [12] Zhang Y, Li Y. New development of Interleukin-6 receptor[J]. Bull Med Res, 2004, 33(1): 37-39. [13] Shizuya K, Komori T, Fujiwara R, et al. The expressions of mRNAs for interleukin-6 (IL-6) and the IL-6 receptor (IL-6R) in the rat hypothalamus and midbrain during restraint stress[J]. Life Sci, 1998, 62(25): 2315-2320. [14] 李改丽,王百忍,段晓莉,等.IL-6受体的多抗制备及其在正常大鼠脑内的分布[J].神经解剖学杂志,2004, 20(3):281-286. [15] Li GL, Wang BR, Duan XL, et al.Antibody preparation and distribution of IL-6 receptor[J]. Chin J Neuroanat, 2004, 20(3): 281-286. [16] Yamashita T, Deguchi K, Sawamoto K, et al. Neuroprotection and neurosupplementation in ischaemic brain[J]. Biochem Soc Trans, 2006, 34(6): 1310-1312.
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