丹参酮IIA对腹膜透析液诱导的大鼠腹膜组织学变化及CTGF表达的影响
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摘要: 目的:;了解丹参酮IIA对腹腔注射4.25%腹膜透析液（PDS）大鼠腹膜组织学变化以及结缔组织生长因子（CTGF）表达的影响。方法:;50只SD雄性大鼠随机分为5组：对照组，每日腹腔注射生理盐水20mL；PDS组，每日腹腔注射4.25% PDS 20mL；丹参酮低、中、高浓度组，每日分别腹腔注射含丹参酮IIA浓度为50mg/L、100mg/L、200mg/L的4.25%PDS20mL。于实验第30天，取壁层腹膜行光镜检查，并用免疫组织化学的方法检测壁层腹膜CTGF的表达情况。结果:; HE、Masson染色显示，在PDS干预下，腹膜显著增厚，胶原沉积显著增多(P<0.01)。与PDS组比较，丹参酮组腹膜明显变薄，胶原沉积明显减少(P<0.01)。在PDS干预下腹膜CTGF表达较对照组显著增加(P<0.01)，丹参酮组CTGF表达与PDS组比较有显著下降(P<0.01)。结论:;丹参酮II A具有抑制葡萄糖PDS导致的实验大鼠腹膜纤维化及CTGF表达增加的作用。
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