紫杉醇作用于肺癌A549细胞核转录因子荧光素酶值变化的意义

安青; 章永红; 刘旭; 桑锋; 金海浩

紫杉醇作用于肺癌A549细胞核转录因子荧光素酶值变化的意义

南京中医药大学第一临床医学院，江苏南京 210046

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出版历程
- 收稿日期: 2011-02-22
- 修回日期: 2011-04-08
- 刊出日期: 2011-05-10

摘要

摘要: 目的研究紫杉醇作用于肺癌A549细胞核转录因子荧光素酶值变化的意义。方法于含10%胎牛血清RPMI1640培养基中，在37℃、5% CO₂条件下细胞培养，转染核转录因子κB（NFκB）及环磷酸腺苷（CREB）质粒，单光子仪测定检测荧光素酶值，激光共聚焦显微镜观察NFκB核易位。结果紫杉醇组NFκB及CREB荧光素酶均值分别为22387±1805、70403±4446明显高于未加药物的空白对照组1967±539、2607±665 (P<0.01)，激光共聚焦显微镜观察到NFκB在紫杉醇作用下由细胞核转出到细胞质内。结论紫杉醇作用于肺癌A549细胞后，核转录因子NFκB及CREB荧光素酶值升高，提示活性增强，紫杉醇可以使NFκB转入到细胞核。
- 紫杉醇 /
- 核转录因子 /
- 肺癌

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