水牛角主要药效学评价及解热活性物质基础研究
Pharmacodynamics research of water buffalo horn and the basic study of water baffulo horn antipyretic active compound
-
摘要: 目的对水牛角的解热、镇静、促凝血等生物活性进行了评价研究，并针对其解热活性的物质基础开展了初步研究。方法采用皮下酵母致热法、光电管法、毛细管法评价了水牛角粉、热提液和冷浸液的解热、镇静、促凝血作用；采用凝胶柱层析法将水牛角热提液按分子量分为大、中、小3个部位。并以皮下酵母致热法评价了各部位的解热作用。结果水牛角粉、水牛角热提液和冷浸液均可降低酵母致热大鼠体温、降低小鼠自发活动次数、缩短小鼠凝血时间，其中热提液的作用强于冷浸液样品；水牛角热提液的大分子量和小分子量部位均能降低酵母致热大鼠体温，中分子量部位作用不明显。结论水牛角粉、水牛角热提液均具有明显的解热、镇静、促凝血作用，冷浸液解热作用较弱；水牛角解热作用的物质基础源于大分子和小分子部位的共同贡献。Abstract: To study the effect of pyretilysis, conscious-sedation, and blood clotting of water buffalo horn, to search the substantial basic of baffulo born. METHOD Pyretolysis experiment, spontaneous movement experiment and blood clotting experiment were used to investigate the bioactivity of water baffulo horn powder, heating water-pheresis and colding water-pheresis. Column chromatography was used to isolate the water baffulo horn heating water-pheresis into three fractions, which were investigated the antipyretic effects. RESULT The experiments showed that water baffulo horn powder, heating water-pberesis and colding water-pberesis, could reduce the times of spontaneous movement, shorten the time of blood clotting time, and reduce the temperature of febrile rat, and the effect of heating water-pheresis was better than colding water-pheresis. The fractions of high-molecular and low-molecular could reduce the temperature of febrile rat, the effect of mid-molecular was inconspicuous. CONCLUSION Water baffulo horn had the effect on pyretolysis, sedation and blood clotting. The fractions of high-molecular and low-molecular had the effects on pyretolysis.
-
[1] 国家药典委员会.中华人民共和国药典·一部[S].北京:化学工业出版社,2005.56. [2] 陈赤.水牛角的研究与应用[J].广西中医学院学报,2004,7(4):72. [3] 金若敏,陈长勋,范广平,等.犀角与水牛角药理作用的研究[J].中成药,1997,19(7):33. [4] Paul Pui-Hay But,Yan-Kit Tam,Lai-Ching Lung.Ethnopharmacology of rhinoceros horn.Ⅱ:antipyretic effects of prescriptions containing rhinoceros horn or water buffalo horn[J].Journal of Ethnopharmacology,1991,33:45. [5] 韩俊艳,檀德宏,王敏伟.水牛角水解物的止血作用研究[J].实用药物与临床.2004,7(3):17. [6] 王斐,段金廒,钱大玮,等.犀角及羚羊角替代资源的寻找与评价研究(Ⅰ)[J].南京中医药大学学报,2005,21(3):163. [7] 王斐,段金廒,钱大玮,等.犀角及羚羊角替代资源的寻找与评价研究(Ⅱ)[J].南京中医药大学学报,2007,23(1):36. [8] 刘天舒.水牛角水提取工艺的研究[J].湖南中医杂志,2005,21(2):77. [9] 李仪奎.中药药理实验方法学[M].上海:上海科学技术出版社,1991:329,493. [10] 徐叔云,卞如濂,陈修.药理实验方法学.第3版[M].北京:人民卫生出版社,2002.934. [11] 王华东,李楚杰,屈洋,等.下丘脑Ca2+、cAMP在家兔EGTA性发热机制中的作用[J].中国病理生理杂志,1996,12(4):402. -
点击查看大图
计量
- 文章访问数: 1102
- HTML全文浏览量: 77
- PDF下载量: 0
- 被引次数: 0
下载:
