基于网络药理学结合设计空间的金银花-连翘药对水提取工艺研究

Research on Water Extraction Process of Flos Lonicerae-Fructus Forsythuae Based on Network Pharmacology and Design Space

    摘要
  • 摘要:
      目的  优选金银花-连翘药对水提取工艺。
      方法  借助网络药理学对金银花-连翘潜在的活性成分进行初步筛选, 再结合《中国药典》2020年版一部金银花、连翘项下规定的质量标志物及参考文献确定指标性成分, 同时以金银花-连翘药对的出膏率及指标性成分的提取率作为水提取工艺的关键质量属性(Critical quality attributes, CQAs), 筛选关键工艺参数(Critical process parameters, CPPs), 通过Box-Behnken设计建立数学模型, 考察CQAs和CPPs之间的交互作用, 构建金银花-连翘水提取工艺的设计空间。
      结果  筛选出酚酸类、连翘酯苷A和连翘苷作为金银花-连翘药对的指标成分, 饮片规格、提取时间、提取加水量为关键工艺参数。基于设计空间确定的最佳水提取工艺参数为: 连翘饮片规格0.8~1.2 cm, 一煎加12倍量水, 提取30~50 min, 二煎加10倍量水, 提取25~30 min。
      结论  运用设计空间验证提取工艺所得的实测值与预测值基本一致, 表明建立的方法稳定可靠, 为金银花-连翘药对的工艺开发及质量控制提供思路。

     

    Abstract:
      OBJECTIVE  To optimize the water extraction process of Flos Lonicerae-Fructus Forsythuae and determine the range of water extraction process parameters.
      METHODS  The active components were screened by network pharmacology, and the indicator ingredients were determined in combination with the quality markers under the relevant terms of Chinese Pharmacopoeia 2020 edition and the literature. Take extraction yield and the extraction rate of the indicative component as the critical quality attributes of the water extraction process to screen critical process parameters. The mathematical model was established by Box-Behnken experimental design to investigate the interaction between CQAs and CPPs and build the design space of the water extraction process of Flos Lonicerae-Fructus Forsythuae.
      RESULTS  The extraction percentages of phenolic acids, forsythoside A and forsythin were screened as the index components; specifications of medicinal slices, extraction time and water addition were the key process parameters. Based on the establishment and optimization of the design space, the optimum water extraction process was obtained as follows: the medicinal slice of Lian-Qiao was broken into 0.8-1.2 cm, adding 12 times the amount of water in the first and extract for 30-50 min, 10 times the amount of water in the second and extract for 25-30 min.
      CONCLUSION  The verification results show that the measured value obtained by using the design space method to optimize the water extraction process is close to the predicted value, indicating that the method is stable and reliable, which can provide ideas for its further process development and quality control for the couple medicines of Flos Lonicerae-Fructus Forsythuae.

     

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