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基于UPLC-QTOF-MS/MS分析的芪葵颗粒化学成分系统筛选与识别

李长印 陆明霞 廖健城 黄莉吉 居文政 邹建东

李长印, 陆明霞, 廖健城, 黄莉吉, 居文政, 邹建东. 基于UPLC-QTOF-MS/MS分析的芪葵颗粒化学成分系统筛选与识别[J]. 南京中医药大学学报, 2023, 39(1): 57-69. doi: 10.14148/j.issn.1672-0482.2023.0057
引用本文: 李长印, 陆明霞, 廖健城, 黄莉吉, 居文政, 邹建东. 基于UPLC-QTOF-MS/MS分析的芪葵颗粒化学成分系统筛选与识别[J]. 南京中医药大学学报, 2023, 39(1): 57-69. doi: 10.14148/j.issn.1672-0482.2023.0057
LI Chang-yin, LU Ming-xia, LIAO Jian-cheng, HUANG Li-ji, JU Wen-zheng, ZOU Jian-dong. Systematic Screening and Identification of Chemical Constituents in Qikui Granules Based on UPLC-QTOF-MS/MS Analysis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(1): 57-69. doi: 10.14148/j.issn.1672-0482.2023.0057
Citation: LI Chang-yin, LU Ming-xia, LIAO Jian-cheng, HUANG Li-ji, JU Wen-zheng, ZOU Jian-dong. Systematic Screening and Identification of Chemical Constituents in Qikui Granules Based on UPLC-QTOF-MS/MS Analysis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(1): 57-69. doi: 10.14148/j.issn.1672-0482.2023.0057

基于UPLC-QTOF-MS/MS分析的芪葵颗粒化学成分系统筛选与识别

doi: 10.14148/j.issn.1672-0482.2023.0057
基金项目: 

江苏省自然科学基金面上项目 BK20201502

“333高层次人才培养工程”科研资助项目 BRA2020384

江苏省中医院高峰人才项目 y2018rc22

江苏省中医院高峰人才项目 y2021rc31

江苏高校优势学科建设工程资助项目 苏政办发〔2018〕87号

南京中医药大学中医学优势学科三期项目开放课题 ZYX03KF030

详细信息
    作者简介:

    李长印, 男, 副研究员, E-mail: fsyy00612@njucm.edu.cn

    通讯作者:

    邹建东, 男, 研究员, 主要从事中医药临床研究数据管理和统计研究, E-mail: jdzou2000@163.com

  • 中图分类号: R284

Systematic Screening and Identification of Chemical Constituents in Qikui Granules Based on UPLC-QTOF-MS/MS Analysis

  • 摘要:   目的  系统定性表征芪葵颗粒(黄芪、制首乌、黄蜀葵花)中的化学成分。  方法  采用超高效液相色谱-四级杆飞行时间质谱(UPLC-QTOF-MS/MS)联用分析方法对芪葵颗粒水提液进行分离检测, 获取正、负离子模式下的原始分析数据; 借助AB Sciex PeakView软件, 综合采用靶向和非靶向的筛选方法, 对获得的原始分析数据进行处理, 系统筛选鉴定芪葵颗粒中的化学成分; 以不少于2个准分子离子的同时出现, 排除碎片离子的干扰, 锁定筛选所得目标化合物; 依据准分子离子及特征二级子离子质谱信息, 结合标准品比对, 并参考数据库搜索匹配和文献数据比对, 对锁定的化合物进行鉴定或合理归属。  结果  从芪葵颗粒中共筛选出89个化合物, 鉴定出71个化合物, 包括黄酮类45个, 皂苷类14个, 蒽醌类4个, 二苯乙烯苷类3个, 多糖类2个, 有机酸类3个。鉴定结果表明羟基化、糖基化、葡萄糖醛酸化、乙酰化、甲基化、丙二酰化等多种转化反应参与了芪葵颗粒多样化学成分的形成。  结论  基于UPLC-QTOF-MS/MS靶向和非靶向相结合的筛选鉴定方法可快速全面表征芪葵颗粒中的主要化学成分及其涉及的转化反应, 为深入探讨芪葵颗粒的质量控制、组方优化和药效物质等奠定了基础。

     

  • 图  1  芪葵颗粒水提液的UPLC-QTOF-MS色谱图

    注:a.负离子模式下TIC图;b.正离子模式下TIC图;c.负离子模式下XIC图;d.正离子模式下XIC图;1~71.已鉴定化学成分LC1~LC71;U1~U18.未鉴定化学成分

    Figure  1.  The UPLC-QTOF-MS chromatograms from the aqueous extract of Qikui Granules

    图  2  毛蕊异黄酮(a)、大黄素甲醚(b)、毛蕊异黄酮苷(c)和大黄素甲醚-8-O-β-D-葡萄糖苷(d)的二级子离子图谱

    注:a和b源自对照品溶液; c和d源自芪葵颗粒水提液, 对应编号为LC14和LC49的目标化合物。

    Figure  2.  Product ion mass spectra of calycosin (a), physcion (b), calycosin-7-O-β-D-glucoside (c) and physcion-8-O-β-D-glucoside (d)

    图  3  芪葵颗粒中主要化学成分的可能化学结构及其可能涉及的转化反应

    注:(1)羟基化; (2)六碳糖基化; (3)葡萄糖醛酸化; (4)乙酰化; (5)去甲基化; (6)甲基化; (7)四碳糖基化; (8)丙二酰化; (9)五碳糖基化; 蓝色表示经标准品比对确认的化合物

    Figure  3.  The candidate structures of the identified compounds and their possible related conversion reactions

    表  1  芪葵颗粒20个主要化学成分对照品信息

    Table  1.   General information of 20 authentic standards for major constituents in Qikui Granules

    编号 名称 生产厂家 批号
    1 芦丁 成都曼斯特生物科技有限公司 MUST-17122001
    2 毛蕊异黄酮苷 中国药品生物制品检定所 111920-201102
    3 二苯乙烯苷 成都曼斯特生物科技有限公司 MUST-18051611
    4 金丝桃苷 成都曼斯特生物科技有限公司 MUST-17101605
    5 异槲皮苷 上海源叶生物科技有限公司 P25J9F65872
    6 棉纤维素 上海源叶生物科技有限公司 Z14M8H30302
    7 棉皮苷 上海源叶生物科技有限公司 T01J11Z117171
    8 杨梅素 上海源叶生物科技有限公司 YM0311YA13
    9 棉皮素 法国EXTRASYNTHESE公司 14110601
    10 刺芒柄花苷 上海源叶生物科技有限公司 P03A10F84910
    11 毛蕊异黄酮 上海源叶生物科技有限公司 Y05J11H115098
    12 黄芪紫檀烷苷 上海源叶生物科技有限公司 P21N9F75533
    13 槲皮素 成都曼斯特生物科技有限公司 MUST-17101104
    14 黄芪异黄烷苷 成都曼斯特生物科技有限公司 MUST-19102620
    15 芒柄花素 中国药品生物制品检定所 111703-200602
    16 黄芪甲苷 中国药品生物制品检定所 0781-200109
    17 黄芪紫檀烷 上海源叶生物科技有限公司 Y28M10H84302
    18 黄芪异黄烷 上海源叶生物科技有限公司 Y28M10H84303
    19 大黄素甲醚 中国药品生物制品检定所 110758-201013
    20 大黄素 南京聚康医药化工有限公司 181215
    下载: 导出CSV

    表  2  芪葵颗粒中鉴定出的71个化合物的详细信息

    Table  2.   The detailed information of 71 identified compounds in Qikui Granules

    编号 tR/min 分子式 [M-H]- [M+HCO OH-H]- [M+HN O3-H]- [M+H]+ [M+Na]+ 强度/ cps 主要特征子离子m/z(峰面积/%) 化合物名称 来源
    LC1 1.16 C12H22O11 341.108 8 (-0.4) 387.113 6 (0.7) 404.103 5 (0.1) 343.123 7 (0.7) 365.105 6 (-1) 387 105 341.110 7(18.15), 179.056 4(6.29), 143.036 4(2.22), 119.035 4(10.81), 113.024 6(5.53), 101.024 8(5.47), 89.025 3(16.27), 71.015 9(8.32) 蔗糖(Sucrose)/松二糖(Turanose)# 1, 2, 3
    LC2 1.27 C6H8O7 191.019 9 (0.8) 193.034 2 (-0.4) 215.016 2 (-2.9) 953 702 191.018 7(6.46), 129.019 5(3.43), 111.009 2(25.4), 87.009 9(23.51), 85.030 7(14.9), 67.021 1(5.12), 57.037 6(3.94), 41.007 0(1.98) 异柠檬酸盐(Isocitrate)/柠檬酸(Citrate)# 1, 2, 3
    LC3 1.51 C6H8O7 191.020 0 (1.2) 193.034 3 (0.2) 215.016 2 (-2.9) 826 587 191.020 6(5.2), 129.019 8(2.27), 111.010 1(25.81), 87.010 5(27.44), 85.031 3(14.15), 67.021 3(6.85), 57.038 2(4.44), 41.007 4(2.57) 异柠檬酸盐(Isocitrate)/柠檬酸(Citrate)# 1, 2, 3
    LC4 1.71 C7H6O5 169.014 4 (1.1) 168 945 169.014 7(15.2), 125.025 0(42.59), 124.016 5(5.23), 97.030 6(2.99), 81.036 1(3.61), 79.020 0(9.26), 69.036 5(2.99), 51.027 3(2.59) 没食子酸(Gallic acid)# 3
    LC5 8.28 C27H28O19 655.114 9 (-0.4) 657.129 7 (0) 679.113 7 (3) 33 921 655.114 8(52.8), 479.082 8(26.2), 316.022 0(9.59), 287.0194 4(1.14), 271.022 3(1.06) 棉皮苷-3-O-葡萄糖苷(Gossypetin-8-O-β-D-glu-3-O-glc) 2
    LC6 9.85 C26H32O14 567.171 6 (-0.5) 613.176 1 (-0.3) 630.165 7 (-1.2) 14 460 613.160 4(1.8), 567.167 0(6.93), 405.118 1(58.83)3, 243.065 8(26.55) 二苯乙烯苷-O-葡萄糖苷(2, 3, 5, 4'-THDPE-2-O-glc-O-glc) 3
    LC7 10.17 C23H28O11 479.155 4 (-1.1) 525.160 0 (-0.5) 542.150 3 (-0.2) 503.154 1 (3.3) 7 166 525.165 9(20.22), 479.162 0(9.62), 449.147 2(36.87), 431.128 3(2.85), 327.111 0(9.19), 165.054 5(2.77), 121.029 0(14.27), 77.041 2(2.72) 羟基黄芪异黄烷苷(Hydroxy-isomucronulatol-7-O-β-D-glc) 1
    LC8 10.92 C21H20O13 479.082 5 (-1.2) 542.076 9 (-1.5) 481.097 6 (-0.1) 503.079 0 (-1.2) 20 568 479.084 2(44.7), 317.026 4(4.75), 316.020 4(29.83), 287.020 9(2.61), 271.024 5(7.13), 179.002 4(1.37) 棉皮素-3-O-半乳糖苷(Gossypetin-3-O-galactoside) 2
    LC9 11.19 C21H20O13 479.082 5 (-1.4) 525.087 6 (0.2) 542.078 0 (0.6) 481.097 7 (0.2) 45 526 479.083 9(47.25), 317.027 9(5.25), 316.021 5(25.36), 287.016 5(4.15), 271.023 7(6.85), 214.024 3(1.23), 151.005 9(0.48) 棉皮素-3-O-吡喃葡萄糖苷(Gossypetin-3-O-glucopyranoside) 2
    LC10 11.59 C26H28O16 595.130 0 (-0.8) 658.125 5 (0.8) 597.144 6 (-0.7) 619.127 4 (0.8) 40 051 595.134 0(56.53), 301.035 9(2.18), 300.028 1(23.84), 271.023 0(4.34), 255.031 9(3.5) 槲皮素-3-O-葡萄糖苷-戊糖苷(Quercetin-3-O-glc-pentose) 2
    LC11 11.70 C22H22O10 491.118 7 (0.6) 508.109 1 (1) 447.128 9 (0.8) 469.109 6 (-1.9) 2 865 445.108 0(36.77), 283.063 4(30.91), 268.035 0(7.71), 267.029 5(12.32), 239.034 2(2.91) 毛蕊异黄酮葡萄糖苷(Calycosin-O-glc) 1
    LC12 12.46 C26H32O14 567.176 1 (-0.6) 613.175 8 (-0.8) 630.166 5 (0) 569.186 2 (-0.6) 591.168 4 (-0.1) 18 935 567.171 5(64.47), 243.062 9(22.8) 四羟基二苯乙烯-2-O-双葡萄糖苷(2, 3, 5, 4'-THDPE-2-O-diglc) 3
    LC13* 12.55 C27H30O16 609.145 2 (-1.6) 672.140 2 (-0.6) 611.161 3 (1.1) 633.141 9 (-1.1) 84 754 609.147 2(51.33), , 300.026 7(22.37), 271.026 2(4.39), 255.031 5(2.02), 178.998 5(0.83), 151.005 2(1.21), 121.030 6(0.16) 芦丁(Rutin)# 2
    LC14* 12.69 C22H22O10 491.118 5 (0.2) 508.108 3 (-0.6) 447.129 0 (0.9) 469.111 0 (1) 217 163 491.117 3(9.13), 283.061 2(52.44), 268.038 0(23.8), 267.033 2(1.22) 毛蕊异黄酮苷(Calycosin-7-O-β-D-glc)# 1
    LC15* 12.75 C20H22O9 405.118 7 (-1.1) 451.123 4 (-0.3) 468.113 4 (-0.6) 429.115 9 (0.7) 1 420 070 405.120 8(6.09), 243.067 1(37.07), 225.056 0(2.68), 215.070 4(1.53), 173.061 0(4.44), 145.065 7(1.7), 137.025 2(4.21), 131.049 6(1.59), 109.029 9(2.25), 93.035 2(2.19) 二苯乙烯苷(2, 3, 5, 4'-THDPE-2-O-glc)# 3
    LC16* 12.90 C21H20O12 463.087 2 (-2.1) 526.082 9 (0.2) 465.102 8 (0.2) 487.084 7 (0.1) 276 602 463.087 6(24.63), 301.034 7(14.76), 300.026 7(25.35), 271.023 4(12.69), 255.028 4(6.12), 243.029 3(2.36), 151.001 7(1.59) 金丝桃苷(Hyperoside) 2
    LC17* 13.21 C21H20O12 463.087 2 (-2.3) 526.082 9 (0.2) 465.102 6 (-0.3) 284 469 463.086 5(35.33), 301.035 3(12.76), 300.025 1(21.97), 271.022 9(11.77), 255.030 2(4.74), 243.025 4(1.29), 178.994 3(0.71), 151.001 7(1.38) 异槲皮苷(Isoquercitrin)# 2
    LC18 13.34 C28H32O15 607.166 4 (-0.8) 653.172 2 (1.5) 670.159 2 (-3.3) 609.182 9 (2.4) 631.164 3 (1.5) 7 650 607.164 7(42.25), 413.108 0(4.17), 193.049 2(33.39), 178.023 5(5.02), 137.023 7(6.41), 134.041 8(2.38), 93.034 0(1.32) 毛蕊异黄酮双葡萄糖苷异构体(Calycosin-diglc isomer) 12
    LC19* 13.47 C21H20O13 479.081 9 (-2.6) 542.077 2 (-0.8) 43 035 479.084 4(53.82), 317.030 7(24.34), 299.009 8(0.26), 271.025 8(2.68), 178.999 3(6.88), 151.004 8(4.21), 137.024 7(1.39) 棉纤维素(Gossypin) 2
    LC20 13.58 C21H20O10 431.096 9 (-3.4) 477.102 4 (-0.7) 494.092 6 (-0.6) 433.113 0 (0.3) 8 577 431.097 6(51.75), 269.044 2(34.54), 268.038 9(10.77) 4'-去甲基毛蕊异黄酮苷(4'-Demethylcalycosin-7-O-β-D-glc) 1
    LC21 14.31 C22H22O11 507.113 0 (-0.7) 524.102 9 (-1.2) 463.124 8 (2.8) 2 782 507.124 4(9.94), 461.106 1(37.93), 446.078 9(2.66), 299.054 9(29.52), 298.047 3(6.53), 284.032 9(2.13), 255.031 5(4.03) 5-羟基毛蕊异黄酮苷(5-Hydroxy-calycosin-7-O-β-D-glc) 1
    LC22* 14.48 C21H18O14 493.061 5 (-1.9) 495.077 3 (0.7) 517.059 1 (0.5) 310 887 493.062 7(22.69), 317.030 6(39.6), 299.021 6(2.21), 271.024 7(1.79), 194.994 0(2.13), 166.998 1(3.85), 139.003 7(2.7) 棉皮苷(Gossypetin-8-O-β-D-glu) 2
    LC23 14.71 C31H40O16 667.223 8 (-0.9) 730.221 6 (3.7) 669.240 9 (2.9) 2 772 667.226 0(64.47), 505.154 4(7.29), 417.155 3(19.88), 181.050 7(2.91), 87.010 3(3.03) 丙二酰黄芪异黄烷苷-四碳糖苷(Malonyl-isomucronulatol-7-O-β-D-glc-tetrose) 1
    LC24 14.78 C22H22O12 477.102 8 (-2.2) 540.098 2 (-0.4) 1 350 477.106 5(29.28), 314.045 6(31.68) 5, 2'-双羟基毛蕊异黄酮苷(5, 2'-Dihydroxy-calycosin-7-O-β-D-glc) 1
    LC25* 14.96 C15H10O8 317.030 0 (-0.9) 380.024 9 (0.1) 319.044 8 (-0.1) 1 829 - 杨梅素(Myricetin) 2
    LC26 14.96 C29H38O15 625.212 5 (-2.1) 671.217 9 (-0.4) 688.208 8 (0.6) 627.231 2 (4.5) 649.211 0 (1) 5 890 625.223 8(2.56), 625.198 9(8.05), 463.159 9(41.85), 301.105 8(28.16), 286.095 6(9.27) 黄芪异黄烷-7, 2'-双葡萄糖苷(Isomucronulatol-7, 2'-diglc) 1
    LC27 15.10 C22H22O11 461.107 3 (-3.6) 507.113 3 (-0.1) 524.103 0 (-1) 463.123 9 (0.9) 18 771 507.105 8(3.05), 461.104 4(3.62), 446.102 9(2.55), 341.066 1(2.23), 299.054 7(61.02), 284.033 0(26.07), 255.027 3(1.46) 2'-羟基毛蕊异黄酮苷(2'-Hydroxy-calycosin-7-O-β-D-glc) 1
    LC28 15.12 C22H24O10 447.128 6 (-2.3) 493.133 7 (-0.7) 510.124 2 (-0.1) 449.143 0 (-2.7) 471.126 5 (0.8) 3 906 493.065 2(13.39), 447.116 4(16.51), 405.125 3(1.43), 317.031 4(1.26), 285.074 6(34.98), 285.042 7(3.59), 270.053 4(25.83) 9-去甲基黄芪紫檀烷苷(9-Demethyl-9, 10-DiMOPTC-3-O-β-D-glc) 1
    LC29* 15.25 C15H10O8 317.030 2 (-0.4) 380.024 1 (-1.9) 38 192 317.031 0(51.85), 193.021 0(1.96), 179.002 3(10.64), 151.000 3(11.29), 137.025 7(9.64), 109.030 7(7.41), 107.013 1(3.65) 棉皮素(Gossypetin) 2
    LC30 15.66 C12H22O11 341.108 0 (-2.8) 387.113 1 (-0.5) 404.103 0 (-1.3) 37 837 - 蔗糖(Sucrose)/松二糖(Turanose) 1, 2, 3
    LC31 15.79 C21H20O12 463.087 5 (-1.5) 509.092 3 (-0.6) 526.082 4 (-0.6) 465.102 7 (-0.2) 487.085 4 (1.5) 165 903 463.090 1(36.5), 301.035 8(40.31), 300.028 9(1.14), 273.033 9(1.1), 178.999 1(5.09), 151.004 2(9.57), 121.029 6(1.94), 107.014 4(1.41) 槲皮素-3'-O-葡萄糖苷(Quercetin-3'-O-glc)# 2
    LC32 15.80 C22H24O10 447.129 4 (-0.6) 493.132 0 (-4.2) 510.121 7 (-4.9) 449.143 6 (-1.5) 471.127 1 (2) 1 454 464.089 0(57.26), 302.039 3(17.51), 301.032 2(10.05), 273.028 4(0.61), 178.996 6(3.23), 152.003 4(2.06), 151.002 5(4.79), 61.989 6(1.61) 10-去甲基黄芪紫檀烷苷(10-Demethyl-9, 10-DiMOPTC-3-O-β-D-glc) 1
    LC33* 16.54 C22H22O9 429.118 7 (-1.1) 475.123 0 (-1) 492.113 2 (-0.9) 431.133 9 (0.7) 453.115 9 (0.6) 185 601 475.124 8(11.91), 267.066 1(62.97), 252.043 1(15.04), 224.046 8(0.34), 223.036 5(0.56), 195.050 5(0.29), 132.023 4(0.18), 45.001 3(4.66) 芒柄花苷(Formononetin-7-O-β-D-glc)# 1
    LC34 16.68 C24H24O11 487.122 6 (-4.1) 533.128 6 (-0.8) 550.118 7 (-0.8) 489.139 2 (0) 511.123 2 (4.2) 36 288 533.121 4(16.24), 325.067 4(1.12), 285.069 4(3.28), 284.074 1(1.05), 283.059 1(55.1), 268.032 5(16.95), 267.024 3(1.01), 45.001 4(5.25) 3'-乙酰基-毛蕊异黄酮苷(3'-Acetyl-calycosin-7-O-β-D-glc) 1
    LC35 16.84 C28H34O14 593.186 8 (-1.3) 639.191 6 (-0.6) 595.202 6 (0.7) 617.182 5 (-2.5) 8 395 593.189 6(51.94), 505.173 7(12.35), 417.153 9(9.9), 402.133 3(2.54), 387.116 3(3.32), 299.056 4(5.1), 181.051 6(4.25), 87.007 8(3.56) 黄芪紫檀烷苷-戊糖苷(9, 10-DiMOPTC-3-O-β-D-glc-pentose) 1
    LC36 17.02 C28H34O14 593.187 2 (-0.7) 639.192 6 (1) 656.182 2 (0.1) 595.202 5 (0.5) 617.184 6 (0.9) 5 613 593.191 6(68.73), 299.095 7(18.06), 284.073 4(13.2) 黄芪紫檀烷苷-戊糖苷(9, 10-DiMOPTC-3-O-β-D-glc-pentose) 1
    LC37 17.48 C16H12O5 283.061 2 (-0.1) 285.075 5 (-1) 307.058 3 (1.9) 11 503 283.065 8(33.08), 268.039 5(29.79), 239.026 7(3.07), 211.044 4(8.31), 195.049 4(5.6), 183.043 5(2.69), 148.019 1(3.02), 91.020 1(2.85) 毛蕊异黄酮同分异构体(Calycosin isomer) 1
    LC38* 17.51 C23H26O10 461.145 3 (-0.1) 507.149 8 (0.2) 524.139 8 (0) 463.159 6 (-0.6) 485.142 0 (0.4) 131 258 507.152 7(33.88), 461.147 9(3.1), 299.092 6(29.1), 298.297 0(0.79), 284.068 5(5.19), 269.046 7(5.22), 45.001 5(17.84) 黄芪紫檀烷苷(9, 10-DiMOPTC-3-O-β-D-glc) 1
    LC39* 17.76 C15H10O7 301.035 2 (-0.7) 364.029 0 (-2.6) 303.049 4 (-1.7) 325.030 6 (-3.8) 62 825 301.034 9(38.21), 273.043 3(1.48), 178.998 3(8.86), 151.003 3(20.59), 133.028 4(1.5), 121.029 7(5.75), 107.013 6(4.57) 槲皮素(Quercetin)# 2
    LC40* 17.95 C16H12O5 283.061 1 (-0.4) 329.067 0 (4.4) 346.055 8 (0.2) 285.075 5 (-0.8) 307.057 5 (-0.5) 638 440 283.060 8(13.08), 268.037 6(26.72), 240.042 0(2.9), 239.034 3(6.44), 211.040 2(11.84), 195.045 7(2.85), 183.044 4(2.51), 148.016 4(2.3), 135.009 1(2.6), 91.020 3(3.76) 毛蕊异黄酮(Calycosin) 1
    LC41* 18.01 C23H28O10 463.160 8 (-0.3) 509.165 5 (0.3) 526.155 3 (-0.5) 465.175 6 (0.1) 487.157 9 (0.9) 34 853 463.157 2(12.73), 301.106 8(58.23), 286.079 5(3.05), 271.060 9(3.98), 164.046 5(2.96), 135.043 8(4.8), 121.027 4(3.84), 109.028 5(1.42) 黄芪异黄烷苷(Isomucronulatol-7-O-β-D-glc) 1
    LC42 18.39 C21H20O10 431.097 6 (-1.8) 494.093 8 (1.9) 455.093 8 (-2.3) 37 321 431.099 2(48.75), 293.043 0(0.89), 269.044 8(36.39), 241.051 0(2.18), 240.046 0(1.28), 225.058 2(5.66), 210.030 3(0.65) 大黄素-8-O-β-D-葡萄糖苷(Emodin-8-O-β-D-glc)# 3
    LC43 18.92 C22H22O10 491.117 7 (-1.4) 508.107 6 (-1.9) 447.127 5 (-2.5) 2 753 491.117 7(11.87), 445.120 5(2.26), 329.241 2(9.72), 283.061 2(59.08), 268.034 4(10.52) 毛蕊异黄酮葡萄糖苷(Calycosin-O-glc) 1
    LC44 19.49 C15H10O5 269.045 3 (-0.9) 332.038 9 (-3.5) 271.061 1 (3.8) 23 026 269.046 0(62.52), 225.055 5(2.41), 197.059 0(1.13), 183.047 1(1.45), 181.066 7(1.49), 159.045 7(2.08), 107.014 6(1.14), 63.025 5(1.43) 大黄素同分异构体(Emodin isomer) 3
    LC45 19.85 C17H16O6 315.087 2 (-0.7) 378.083 0 (2.6) 317.102 0 (0.1) 339.083 3 (-1.9) 19 835 315.088 0(20.41), 297.039 7(2.25), 285.040 0(2.04), 163.003 2(2.97), 109.030 2(61.88), 67.020 1(1.67), 65.041 7(3.82), 41.007 8(1.41) 2-羟基黄芪紫檀烷(2, 3-Dihydroxy-9, 10-DiMOPTC) 1
    LC46 19.91 C47H78O19 945.503 1 (-3.6) 991.511 5 (0.7) 947.520 8 (-0.2) 969.507 3 (4.5) 4 160 991.518 6(23.87), 946.518 6(9.11), 945.507 3(61.81), 783.492 3(0.98), 783.462 6(0.49), 59.012 9(0.2) 黄芪皂苷Ⅴ/Ⅵ/Ⅶ同分异构体(Astragaloside Ⅴ/Ⅵ/Ⅶ isomer) 1
    LC47 20.00 C16H12O6 299.055 8 (-1) 362.051 1 (1.3) 13 358 299.054 5(34.81), 284.031 3(32.94), 256.036 7(2.99), 255.028 0(4.54), 227.033 1(2.81), 211.035 4(0.9), 199.037 9(1), 183.044 6(1.08), 148.014 2(3.35) 5-羟基毛蕊异黄酮(5-Hydroxy-calycosin) 1
    LC48 20.01 C17H14O5 297.076 8 (-0.2) 343.081 9 (1.9) 321.074 2 (2.8) 6 855 297.079 6(27.68), 282.055 8(13.32), 281.046 6(17.64), 267.028 2(11.44), 253.057 0(5.81), 239.035 9(16.53), 211.038 1(2.65), 195.039 8(1.7), 91.018 6(1.45) 3'-甲基毛蕊异黄酮(3'-Methyl-calycosin) 1
    LC49 20.17 C22H22O10 445.112 8 (-2.7) 491.118 0 (-0.8) 508.107 9 (-1.3) 2 819 491.118 0(6.76), 427.105 7(6.76), 325.074 6(8.84), 283.060 3(64.09), 240.038 0(9.66) 大黄素甲醚-8-O-β-D-葡萄糖苷(Physcion-8-O-β-D-glc) 3
    LC50 20.40 C16H12O6 299.056 0 (-0.4) 345.061 1 (1.7) 362.051 8 (3) 301.070 7 (0.1) 323.051 3 (-3.9) 48 015 299.054 8(29.7), 284.032 2(31.38), 255.025 2(4.54), 239.039 8(1.47), 227.032 4(8.13), 211.040 4(1.53), 199.044 2(4.01), 148.017 0(1.41) 2'-羟基毛蕊异黄酮(2'-Hydroxy-calycosin) 1
    LC51 20.61 C41H68O14 783.453 1 (-0.6) 829.458 4 (0.5) 846.447 6 (-0.7) 785.467 6 (-0.8) 807.450 0 (-0.2) 1 960 830.486 2(0.7), 829.459 1(22.58), 784.472 8(2.74), 783.456 6(71.2), 119.030 7(1.07) 黄芪甲苷同分异构体(Astragaloside Ⅳ isomer) 1
    LC52 20.75 C25H30O11 505.169 7 (-3.6) 551.174 8 (-2.1) 568.165 9 (-0.3) 507.184 7 (-2.7) 529.167 7 (-0.6) 1 325 551.181 4(7.82), 505.168 7(42.31), 301.109 2(35.19), 164.044 5(3.1), 135.042 8(2.29), 121.031 7(2.5) 黄芪异黄烷-乙酰葡萄糖苷(Isomucronulatol-acetyl-glc) 1
    LC53 21.34 C47H78O19 945.504 9 (-1.6) 991.510 9 (0) 969.500 2 (-2.9) 6 507 991.518 7(53.75), 946.511 5(6.66), 945.510 8(24.73), 783.448 6(1.54), 161.046 0(0.32), 119.034 4(0.13) 黄芪皂苷Ⅴ/Ⅵ/Ⅶ同分异构体(Astragaloside Ⅴ/Ⅵ/Ⅶ isomer) 1
    LC54* 21.65 C16H12O4 267.066 2 (-0.3) 330.060 8 (-0.2) 269.080 9 (0.3) 291.062 7 (-0.1) 518 951 267.065 4(15.05), 252.042 3(23.58), 223.040 0(13.26), 208.052 6(1.97), 195.045 0(10.65), 167.050 1(2.44), 135.008 8(2.29), 132.022 0(4.27), 104.027 3(1.27), 91.020 1(3.09) 芒柄花素(Formononetin)# 1
    LC55 21.65 C41H68O14 829.456 5 (-1.9) 846.446 7 (-1.7) 1 377 - 黄芪甲苷同分异构体(Astragaloside Ⅳ isomer) 1
    LC56* 22.07 C41H68O14 829.458 8 (1) 846.449 2 (1.2) 785.468 1 (-0.1) 807.453 8 (4.6) 38 167 829.461 1(80.16), 827.184 2(3.18), 783.462 7(3.57), 179.054 6(0.36), 45.001 5(0.67) 黄芪甲苷(Astragaloside Ⅳ)# 1
    LC57* 22.08 C17H16O5 299.092 3 (-0.7) 345.095 6 (-3.6) 301.107 1 (0.1) 323.088 6 (-1.4) 22 053 299.091 1(15.76), 284.067 4(19.25), 269.045 9(31.61), 241.049 0(9.19), 225.055 7(1.94), 199.037 5(2.03), 185.065 1(1.96), 133.027 6(1.33), 115.055 0(1.39) 黄芪紫檀烷(3-Hydroxy-9, 10-DiMOPTC)# 1
    LC58 22.09 C17H14O5 297.076 9 (0) 299.089 4 (-6.6) 1 713 297.044 2(54.62), 281.020 4(23.18), 251.163 7(10.5), 160.982 1(11.7) 7-甲基毛蕊异黄酮(7-Methyl-calycosin) 1
    LC59 22.26 C41H68O14 783.451 9 (-2.2) 829.458 3 (0.3) 846.448 1 (-0.1) 807.449 9 (-0.3) 17 637 829.461 2(39.31), 783.458 2(52.7), 161.044 1(0.88), 113.023 4(0.6), 101.023 2(0.94) 黄芪甲苷同分异构体(Astragaloside Ⅳ isomer) 1
    LC60* 22.49 C17H18O5 301.107 9 (-0.9) 303.122 9 (0.6) 2 856 301.105 2(30.35), 286.092 6(11.83), 271.065 2(18.24), 256.038 4(11.19), 229.044 9(7.06), 227.074 1(1.76), 149.025 2(6.41), 135.044 0(5.42), 124.015 8(3.9), 121.033 9(3.85) 黄芪异黄烷(Isomucronulatol) 1
    LC61 23.02 C17H16O5 299.091 3 (-4.2) 301.107 5 (1.6) 567 299.093 0(30.09), 284.067 4(31.28), 269.054 5(20.93), 269.042 4(16.17), 171.047 2(1.52) 黄芪紫檀烷异构体(Hydroxy-diMOPTC) 1
    LC62 23.14 C48H78O18 941.511 2 (-0.4) 987.516 5 (0.6) 8 065 987.519 2(6.47), 941.518 5(77.91), 615.392 1(0.33), 512.464 8(0.13), 85.031 8(0.11) 大豆皂苷(Soyasaponin) 1
    LC63 23.29 C43H70O15 871.468 8 (0.3) 888.459 4 (0.8) 827.478 8 (0) 849.461 2 (0.6) 73 219 871.470 3(74.22), 825.462 1(3.98), 765.435 8(1.02), 179.055 4(0.16), 161.048 2(0.2), 113.022 5(0.22), 45.000 8(0.94) 黄芪皂苷Ⅱ同分异构体(Astragaloside Ⅱ isomer) 1
    LC64 24.08 C43H70O15 871.469 4 (0.9) 888.458 7 (-0.1) 827.479 0 (0.3) 849.460 0 (-0.8) 26 480 871.472 7(84.22), 825.471 1(1.15), 765.440 4(0.58), 101.023 2(0.05), 89.023 5(0.09), 59.014 8(0.11), 45.001 5(0.84) 黄芪皂苷Ⅱ同分异构体(Astragaloside Ⅱ isomer) 1
    LC65 24.33 C16H12O5 283.061 1 (-0.5) 285.077 0 (4.4) 7 407 283.060 2(34.59), 268.037 5(28.16), 240.041 6(1.38), 239.035 5(3.99), 211.039 3(4.49), 195.043 1(1.37), 183.045 7(1.44), 135.009 0(0.35), 132.021 7(1.77) 毛蕊异黄酮同分异构体(Calycosin isomer) 1
    LC66 24.68 C43H70O15 871.468 6 (0.1) 888.459 5 (0.8) 827.478 7 (0) 849.460 7 (0) 22 811 871.473 4(80.78), 825.490 9(0.82), 825.468 6(1.34), 825.444 4(0.21), 765.445 4(0.41), 119.036 8(0.12), 89.025 0(0.05), 45.001 0(0.94) 黄芪皂苷Ⅱ同分异构体(Astragaloside Ⅱ isomer) 1
    LC67 25.45 C45H72O16 913.479 4 (0.2) 930.468 7 (-0.6) 869.489 6 (0.4) 891.472 4 (1.2) 60 681 913.485 1(80.09), 867.484 4(1.94), 825.469 8(0.5), 807.450 5(0.13), 119.035 6(0.05), 59.016 6(0.1), 45.001 8(1.04) 黄芪皂苷Ⅰ同分异构体(Astragaloside Ⅰ isomer)# 1
    LC68 26.04 C45H72O16 913.479 0 (-0.2) 930.471 3 (2.1) 869.489 0 (-0.4) 891.473 1 (2.1) 54 160 913.485 1(75.47), 867.521 3(0.25), 867.475 7(1.61), 825.467 3(0.37), 807.448 6(0.82), 89.023 7(0.11), 59.018 4(0.07), 45.001 4(1.06) 黄芪皂苷Ⅰ同分异构体(Astragaloside Ⅰ isomer)# 1
    LC69* 26.16 C15H10O5 269.045 5 (-0.3) 271.058 9 (-4.4) 364 991 269.044 5(37.17), 241.049 3(5.98), 225.054 9(13.29), 210.030 9(2.39), 197.060 7(2.69), 195.044 7(2.7), 185.059 5(2.29), 182.036 1(3.41), 171.044 0(1.41) 大黄素(Emodin) 3
    LC70 26.78 C45H72O16 913.479 9 (0.8) 930.467 7 (-1.8) 869.488 6 (-0.8) 891.469 6
    (-1.9)
    12 211 913.482 8(84.71), 867.468 7(1.74), 825.465 3(0.36), 807.464 2(0.28), 112.986 3(0.05), 89.025 5(0.07), 45.002 2(0.77) 黄芪皂苷Ⅰ同分异构体(Astragaloside Ⅰ isomer)# 1
    LC71 28.00 C47H74O17 955.489 7 (0) 972.478 6 (-1.3) 1 700 955.491 7(81.83), 909.508 8(1.71), 161.038 5(0.1), 146.967 3(0.69), 112.984 8(0.34), 45.000 3(0.84) 乙酰黄芪皂苷Ⅰ(Acetyl astragaloside Ⅰ) 1
    注: *标准品比对确认; #AB Sciex中药高分辨质谱数据库比对; 产生特征碎片离子的准分子离子及其峰强度; 1.黄芪; 2.黄蜀葵花; 3.何首乌; THDPE.四羟基二苯乙烯; HPTC.羟基紫檀烷; MOPTC.甲氧基紫檀烷; glc.葡萄糖; glu.葡萄糖醛酸。
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出版历程
  • 收稿日期:  2021-11-06
  • 网络出版日期:  2023-01-18
  • 发布日期:  2023-01-10

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