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针刺内关和足三里双穴位对大鼠心肌缺血再灌注损伤后的血管重塑作用

张宏如 陈静 徐森磊 顾任钧 白桦 卢圣锋 潘玉璟 顾一煌

张宏如, 陈静, 徐森磊, 顾任钧, 白桦, 卢圣锋, 潘玉璟, 顾一煌. 针刺内关和足三里双穴位对大鼠心肌缺血再灌注损伤后的血管重塑作用[J]. 南京中医药大学学报, 2023, 39(1): 42-49. doi: 10.14148/j.issn.1672-0482.2023.0042
引用本文: 张宏如, 陈静, 徐森磊, 顾任钧, 白桦, 卢圣锋, 潘玉璟, 顾一煌. 针刺内关和足三里双穴位对大鼠心肌缺血再灌注损伤后的血管重塑作用[J]. 南京中医药大学学报, 2023, 39(1): 42-49. doi: 10.14148/j.issn.1672-0482.2023.0042
ZHANG Hong-ru, CHEN Jing, XU Sen-lei, GU Ren-jun, BAI Hua, LU Shen-feng, PAN Yu-jing, GU Yi-huang. The Effect of Combination Acupuncture Treatment of Neiguan and Zusanli on Vascular Remodeling after Myocardial Ischemia Reperfusion Injury[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(1): 42-49. doi: 10.14148/j.issn.1672-0482.2023.0042
Citation: ZHANG Hong-ru, CHEN Jing, XU Sen-lei, GU Ren-jun, BAI Hua, LU Shen-feng, PAN Yu-jing, GU Yi-huang. The Effect of Combination Acupuncture Treatment of Neiguan and Zusanli on Vascular Remodeling after Myocardial Ischemia Reperfusion Injury[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(1): 42-49. doi: 10.14148/j.issn.1672-0482.2023.0042

针刺内关和足三里双穴位对大鼠心肌缺血再灌注损伤后的血管重塑作用

doi: 10.14148/j.issn.1672-0482.2023.0042
基金项目: 

国家自然科学基金面上项目 81974583

南京中医药大学校自然青年基金项目 NZY81704169

详细信息
    通讯作者:

    张宏如, 男, 副教授, 主要从事针灸效应机制研究, E-mail: 170947@njucm.edu.cn

  • 中图分类号: R285.5

The Effect of Combination Acupuncture Treatment of Neiguan and Zusanli on Vascular Remodeling after Myocardial Ischemia Reperfusion Injury

  • 摘要:   目的  研究针刺内关和足三里对心肌缺血再灌注损伤后的血管重塑作用。  方法  将SPF级雄性SD大鼠分为空白组(10只)、MIRI组(10只)、内关组(10只)、足三里组(10只)以及内关+足三里组(10只)。对大鼠进行心肌缺血再灌注(MIRI)处理从而构建MIRI动物模型。穴取双侧内关、足三里以及内关+足三里, 疏密波, 频率2 Hz。关胸后第2天进行电针治疗, 每日1次, 共14 d。超声心动图(ECG)检查各组大鼠左心室射血分数(LVEF)及左心室内径缩短率(LVFS)。TTC染色检测各组大鼠心肌梗死面积。免疫荧光法(IF)检测各组大鼠心肌组织中α-SMA和CD31蛋白共表达情况。蛋白免疫印迹法(Western blot)分别检测各组大鼠心肌组织中VEGFA、α-SMA, CD31和p-eNOS蛋白表达水平变化。  结果  与空白组比较, MIRI模型组大鼠LVEF和LVFS参数显著降低(P < 0.000 1)。针刺内关或足三里穴位的大鼠LVEF和LVFS参数较未进行针刺的MIRI大鼠显著升高(P < 0.000 1)。针刺内关和足三里双穴位的大鼠LVEF和LVFS参数相比MIRI模型组(P < 0.000 1)以及针刺单穴位组(P < 0.01)均有显著升高。MIRI组的梗死面积显著高于空白组(P < 0.000 1)。单独针刺内关穴(P < 0.001)和足三里穴(P < 0.01)能显著缩小梗死面积, 而针刺内关穴和足三里双穴位相比针刺单个穴位能更有效缩小梗死面积(P < 0.05, P < 0.001)。与空白组比较, MIRI组大鼠心肌组织中α-SMA和CD31表达阳性显著减弱。相较MIRI组, 单独针刺内关或足三里穴位组的大鼠心肌组织中α-SMA和CD31表达阳性增强, 而针刺内关和足三里双穴位组的表达阳性强于MIRI对照组以及针刺单穴位组。MIRI组的α-SMA、CD31、VEGFA和p-eNOS蛋白表达水平相对空白组显著降低(P < 0.000 1)。单独针刺内关或足三里穴位组的大鼠心肌组织中VEGFA(P < 0.001, P < 0.000 1)、α-SMA(P < 0.000 1)、CD31(P < 0.000 1)和p-eNOS(P < 0.000 1)表达增强, 而针刺内关和足三里双穴位组表达升高更为显著(P < 0.000 1)。  结论  针刺内关和足三里双穴位对心肌缺血再灌注损伤后心血管重塑效果更佳。

     

  • 图  1  超声心动图分析模前后大鼠的心电情况

    Figure  1.  Detect the electrocardiogram of rats in each group by ECG

    图  2  超声心动图检测各组大鼠的LVEF及LVFS (x±s, n=10)

    注: **P < 0.01, ****P < 0.000 1。

    Figure  2.  Detect the LVEF and LVFS by ECG (x±s, n=10)

    图  3  TTC染色检测各组大鼠心肌梗死面积(x±s, n=10)

    注: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1。

    Figure  3.  Detect the infarct area in each groups using TTC staining (x±s, n=10)

    图  4  免疫荧光法检测各组大鼠心肌组织中CD31和α-SMA共表达情况

    Figure  4.  Test the co-expression of α-SMA and CD31 protein in each groups by IF

    图  5  Western blot法检测各组大鼠心肌组织中VEGFA、CD31、α-SMA和p-eNOS蛋白表达变化

    注: ***P < 0.001, ****P < 0.000 1。

    Figure  5.  Measure the protein levels of VEGFA, α-SMA and CD31 in each groups by Western blot

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  • 收稿日期:  2022-11-20
  • 网络出版日期:  2023-01-18
  • 发布日期:  2023-01-10

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