芪地明目颗粒对糖尿病大鼠视网膜损伤的保护作用

Protective Effect of Qidi Mingmu Granules on Retinal Injury in Diabetic Rats

    摘要
  • 摘要:
      目的  观察芪地明目颗粒对糖尿病视网膜病变(DR)大鼠视网膜组织及血管损伤的保护作用, 探讨其改善DR的可能机制。
      方法  选择SD雄性大鼠以高脂饲料喂养配合STZ注射建立DR模型, 用低、高剂量(1.0、3.0 g · kg-1 · d-1)芪地明目颗粒分别进行干预, 并设立正常对照组、DR模型组及羟苯磺酸钙干预组, 观察并比较干预后各组视网膜组织的病理改变、微血管数目、内皮细胞与周细胞比值(E/P比值), 以及视网膜组织中TGF-β1、miR-200b、VEGF表达水平的差异。
      结果  芪地明目颗粒低、高剂量组及羟苯磺酸钙组的视网膜组织病理改变较DR组明显减轻, 视网膜微血管数目及E/P比值较DR组均显著下降(P < 0.01), TGF-β1、VEGF蛋白及mRNA表达水平均显著下降(P < 0.05), miR-200b表达水平显著提高(P < 0.05)；芪地明目颗粒高剂量干预组的作用明显优于低剂量组(P < 0.05)。
      结论  芪地明目颗粒可减轻DR大鼠视网膜损伤, 抑制视网膜微血管增殖。其可能通过抑制视网膜中TGF-β1/miR-200b/VEGF信号通路激活而达到对DR的改善作用。

     

    Abstract:
      OBJECTIVE  To observe the protective effect of Qidi Mingmu granules on retinal tissue and vascular injury in diabetic retinopathy (DR) rats, and explore the possible mechanism of treating DR.
      METHODS  Male SD rats were selected to establish DR models by high-fat diet and streptozotocin. Low-dose and high-dose(1.0, 3.0 g · kg-1 · d-1) Qidi Mingmu granules were respectively used for DR rats by gavage, and normal control group, DR group (with no treatment) and Calcium Dobesilate treating group were also set up. The pathological changes of retinal tissues, the retinal vascular quantity, the ratio of endothelial cells to pericytes (E/P ratio) and the expression levels of TGF-β1, miR-200b and VEGF in retinal tissues were observed and compared in each group after intervention.
      RESULTS  Compared with DR group, the retinal pathological changes of low-dose and high-dose Qidi Mingmu granules groups and Calcium Dobesilate treating group were significantly improved, the retinal vascular quantity and E/P ratio were significantly decreased (P < 0.01), the protein and mRNA expression levels of TGF-β1 and VEGF significantly decreased (P < 0.05), and the miR-200b expression level significantly increased (P < 0.05). In addition, the effect of Qidi Mingmu granules on DR rats became more significant with the increase of dose (P < 0.05).
      CONCLUSION  Qidi Mingmu granules can relieve retinal injury and inhibit retinal vascular proliferation in DR rats. It may improve DR by inhibiting the activation of TGF-β1/miR-200b/VEGF pathway in retina.

     

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